TY - JOUR
T1 - Improving extracellular vesicles visualization
T2 - From static to motion
AU - Reclusa, Pablo
AU - Verstraelen, Peter
AU - Taverna, Simona
AU - Gunasekaran, Muthukumar
AU - Pucci, Marzia
AU - Pintelon, Isabel
AU - Claes, Nathalie
AU - de Miguel-Pérez, Diego
AU - Alessandro, Riccardo
AU - Bals, Sara
AU - Kaushal, Sunjay
AU - Rolfo, Christian
N1 - Funding Information:
Marzia Pucci is supported by a “AIRC” (Associazione Italiana Ricerca sul Cancro) fellowship. “The Leica SP 8 (Hercules grant AUHA.15.12) microscope was funded by the Hercules Foundation of the Flemish Government”. DdM-P is funded by the University of Granada PhD grant and University of Granada international mobility grant 2018/19.
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - In the last decade, extracellular vesicles (EVs) have become a hot topic. The findings on EVs content and effects have made them a major field of interest in cancer research. EVs, are able to be internalized through integrins expressed in parental cells, in a tissue specific manner, as a key step of cancer progression and pre-metastatic niche formation. However, this specificity might lead to new opportunities in cancer treatment by using EVs as devices for drug delivery. For future applications of EVs in cancer, improved protocols and methods for EVs isolation and visualization are required. Our group has put efforts on developing a protocol able to track the EVs for in vivo internalization analysis. We showed, for the first time, the videos of labeled EVs uptake by living lung cancer cells.
AB - In the last decade, extracellular vesicles (EVs) have become a hot topic. The findings on EVs content and effects have made them a major field of interest in cancer research. EVs, are able to be internalized through integrins expressed in parental cells, in a tissue specific manner, as a key step of cancer progression and pre-metastatic niche formation. However, this specificity might lead to new opportunities in cancer treatment by using EVs as devices for drug delivery. For future applications of EVs in cancer, improved protocols and methods for EVs isolation and visualization are required. Our group has put efforts on developing a protocol able to track the EVs for in vivo internalization analysis. We showed, for the first time, the videos of labeled EVs uptake by living lung cancer cells.
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U2 - 10.1038/s41598-020-62920-0
DO - 10.1038/s41598-020-62920-0
M3 - Article
C2 - 32300120
AN - SCOPUS:85083631807
SN - 2045-2322
VL - 10
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 6494
ER -
