TY - JOUR
T1 - Improving the quality and reproducibility of flow cytometry in the lung
AU - Tighe, Robert M.
AU - Misharin, Alexander V.
AU - Jakubzick, Claudia V.
AU - Brinkman, Ryan
AU - Curtis, Jeffrey L.
AU - Duggan, Ryan
AU - Freeman, Christine M.
AU - Herold, Susanne
AU - Janssen, William
AU - Nakano, Hideki
AU - Redente, Elizabeth F.
AU - Singer, Benjamin D.
AU - Sperling, Anne I.
AU - Swaminathan, Suchitra
AU - Yu, Yen Rei
AU - Zacharias, William J.
N1 - Funding Information:
Supported by National Institutes of Health grants K08HL128867 and U19AI135964 (B.D.S.); Department of Veterans Affairs grant 1IK2BX002401 (E.F.R.); Department of Veterans Affairs grant I01 CX000911 (J.L.C.); National Institutes of Health/National Heart, Lung, and Blood Institute grant U01HL137880 (J.L.C.); German Research Foundation grant KFO309 project 284237345, SFB1021 project 197785619, and EXC2026 project 390649896 (S.H.); National Institutes of Health grants HL135124, AG049665, AI135964, and Department of Defense grant PR141319 (A.V.M.); National Institutes of Health grants R01ES027574, R01ES028829, and K08HL105537 (R.M.T.); and National Institutes of Health grants R35HL140039 and R01HL130938 (W.J.J.).
Publisher Copyright:
© 2019 American Thoracic Society. All rights reserved.
PY - 2019/8/1
Y1 - 2019/8/1
N2 - Defining responses of the structural and immune cells in biologic systems is critically important to understanding disease states and responses to injury. This requires accurate and sensitive methods to define cell types in organ systems. The principal method to delineate the cell populations involved in these processes is flow cytometry. Although researchers increasingly use flow cytometry, technical challenges can affect its accuracy and reproducibility, thus significantly limiting scientific advancements. This challenge is particularly critical to lung immunology, as the lung is readily accessible and therefore used in preclinical and clinical studies to define potential therapeutics. Given the importance of flow cytometry in pulmonary research, the American Thoracic Society convened a working group to highlight issues and technical challenges to the performance of high-quality pulmonary flow cytometry, with a goal of improving its quality and reproducibility.
AB - Defining responses of the structural and immune cells in biologic systems is critically important to understanding disease states and responses to injury. This requires accurate and sensitive methods to define cell types in organ systems. The principal method to delineate the cell populations involved in these processes is flow cytometry. Although researchers increasingly use flow cytometry, technical challenges can affect its accuracy and reproducibility, thus significantly limiting scientific advancements. This challenge is particularly critical to lung immunology, as the lung is readily accessible and therefore used in preclinical and clinical studies to define potential therapeutics. Given the importance of flow cytometry in pulmonary research, the American Thoracic Society convened a working group to highlight issues and technical challenges to the performance of high-quality pulmonary flow cytometry, with a goal of improving its quality and reproducibility.
KW - Cells
KW - Flow cytometry
KW - Lung biology
KW - Reproducibility
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U2 - 10.1165/rcmb.2019-0191ST
DO - 10.1165/rcmb.2019-0191ST
M3 - Article
C2 - 31368812
AN - SCOPUS:85070916543
SN - 1044-1549
VL - 61
SP - 150
EP - 161
JO - American journal of respiratory cell and molecular biology
JF - American journal of respiratory cell and molecular biology
IS - 2
ER -