Improving the quality and reproducibility of flow cytometry in the lung

Robert M. Tighe*, Alexander V. Misharin, Claudia V. Jakubzick, Ryan Brinkman, Jeffrey L. Curtis, Ryan Duggan, Christine M. Freeman, Susanne Herold, William Janssen, Hideki Nakano, Elizabeth F. Redente, Benjamin D. Singer, Anne I. Sperling, Suchitra Swaminathan, Yen Rei Yu, William J. Zacharias

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Defining responses of the structural and immune cells in biologic systems is critically important to understanding disease states and responses to injury. This requires accurate and sensitive methods to define cell types in organ systems. The principal method to delineate the cell populations involved in these processes is flow cytometry. Although researchers increasingly use flow cytometry, technical challenges can affect its accuracy and reproducibility, thus significantly limiting scientific advancements. This challenge is particularly critical to lung immunology, as the lung is readily accessible and therefore used in preclinical and clinical studies to define potential therapeutics. Given the importance of flow cytometry in pulmonary research, the American Thoracic Society convened a working group to highlight issues and technical challenges to the performance of high-quality pulmonary flow cytometry, with a goal of improving its quality and reproducibility.

Original languageEnglish (US)
Pages (from-to)150-161
Number of pages12
JournalAmerican journal of respiratory cell and molecular biology
Volume61
Issue number2
DOIs
StatePublished - Aug 1 2019

Keywords

  • Cells
  • Flow cytometry
  • Lung biology
  • Reproducibility

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

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