In antigen-specific induction of IL-22 in human subjects that resolved HCV infection

Matthew F. Cusick*, Jane E. Libbey, Robert S. Fujinami, David D. Eckels

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Aims: To determine if in vitro production of IL-22 and IL-17 correlated with resolution of HCV infection. Materials & methods: Human peripheral blood cells isolated from a well-defined cohort of resolved and chronic HCV-infected subjects were used to measure HCV-, influenza- and mitogen-activated T-cell proliferation. In addition, IL-22 and IL-17 production was measured via ELISAs and flow cytometry. Results: Resolved HCV subjects had a significantly higher T-cell proliferative response to recombinant NS3 protein compared with chronic HCV subjects. Resolved subjects had a dose-dependent IL-22 response to recombinant NS3 compared with chronic HCV subjects. Conclusion: IL-22 production is associated with antigen-specific induction of CD4 + T cells in individuals that resolved HCV infection, suggesting a potential role for IL-22 in HCV clearance.

Original languageEnglish (US)
Pages (from-to)719-728
Number of pages10
JournalFuture Virology
Volume7
Issue number7
DOIs
StatePublished - Jul 2012

Keywords

  • IL-17
  • IL-22
  • T cell
  • hepatitis C virus
  • human PBMC

ASJC Scopus subject areas

  • Virology

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