In antigen-specific induction of IL-22 in human subjects that resolved HCV infection

Matthew F. Cusick; Jane E. Libbey; Robert S. Fujinami; David D. Eckels

doi:10.2217/fvl.12.58

In antigen-specific induction of IL-22 in human subjects that resolved HCV infection

Matthew F. Cusick^*, Jane E. Libbey, Robert S. Fujinami, David D. Eckels

^*Corresponding author for this work

Surgery, Transplant Surgery Division

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Aims: To determine if in vitro production of IL-22 and IL-17 correlated with resolution of HCV infection. Materials & methods: Human peripheral blood cells isolated from a well-defined cohort of resolved and chronic HCV-infected subjects were used to measure HCV-, influenza- and mitogen-activated T-cell proliferation. In addition, IL-22 and IL-17 production was measured via ELISAs and flow cytometry. Results: Resolved HCV subjects had a significantly higher T-cell proliferative response to recombinant NS3 protein compared with chronic HCV subjects. Resolved subjects had a dose-dependent IL-22 response to recombinant NS3 compared with chronic HCV subjects. Conclusion: IL-22 production is associated with antigen-specific induction of CD4 ⁺ T cells in individuals that resolved HCV infection, suggesting a potential role for IL-22 in HCV clearance.

Original language	English (US)
Pages (from-to)	719-728
Number of pages	10
Journal	Future Virology
Volume	7
Issue number	7
DOIs	https://doi.org/10.2217/fvl.12.58
State	Published - Jul 2012

Keywords

IL-17
IL-22
T cell
hepatitis C virus
human PBMC

ASJC Scopus subject areas

Virology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2217/fvl.12.58

Cite this

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title = "In antigen-specific induction of IL-22 in human subjects that resolved HCV infection",

abstract = "Aims: To determine if in vitro production of IL-22 and IL-17 correlated with resolution of HCV infection. Materials & methods: Human peripheral blood cells isolated from a well-defined cohort of resolved and chronic HCV-infected subjects were used to measure HCV-, influenza- and mitogen-activated T-cell proliferation. In addition, IL-22 and IL-17 production was measured via ELISAs and flow cytometry. Results: Resolved HCV subjects had a significantly higher T-cell proliferative response to recombinant NS3 protein compared with chronic HCV subjects. Resolved subjects had a dose-dependent IL-22 response to recombinant NS3 compared with chronic HCV subjects. Conclusion: IL-22 production is associated with antigen-specific induction of CD4 + T cells in individuals that resolved HCV infection, suggesting a potential role for IL-22 in HCV clearance.",

keywords = "IL-17, IL-22, T cell, hepatitis C virus, human PBMC",

author = "Cusick, {Matthew F.} and Libbey, {Jane E.} and Fujinami, {Robert S.} and Eckels, {David D.}",

year = "2012",

month = jul,

doi = "10.2217/fvl.12.58",

language = "English (US)",

volume = "7",

pages = "719--728",

journal = "Future Virology",

issn = "1746-0794",

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T1 - In antigen-specific induction of IL-22 in human subjects that resolved HCV infection

AU - Cusick, Matthew F.

AU - Libbey, Jane E.

AU - Fujinami, Robert S.

AU - Eckels, David D.

PY - 2012/7

Y1 - 2012/7

N2 - Aims: To determine if in vitro production of IL-22 and IL-17 correlated with resolution of HCV infection. Materials & methods: Human peripheral blood cells isolated from a well-defined cohort of resolved and chronic HCV-infected subjects were used to measure HCV-, influenza- and mitogen-activated T-cell proliferation. In addition, IL-22 and IL-17 production was measured via ELISAs and flow cytometry. Results: Resolved HCV subjects had a significantly higher T-cell proliferative response to recombinant NS3 protein compared with chronic HCV subjects. Resolved subjects had a dose-dependent IL-22 response to recombinant NS3 compared with chronic HCV subjects. Conclusion: IL-22 production is associated with antigen-specific induction of CD4 + T cells in individuals that resolved HCV infection, suggesting a potential role for IL-22 in HCV clearance.

AB - Aims: To determine if in vitro production of IL-22 and IL-17 correlated with resolution of HCV infection. Materials & methods: Human peripheral blood cells isolated from a well-defined cohort of resolved and chronic HCV-infected subjects were used to measure HCV-, influenza- and mitogen-activated T-cell proliferation. In addition, IL-22 and IL-17 production was measured via ELISAs and flow cytometry. Results: Resolved HCV subjects had a significantly higher T-cell proliferative response to recombinant NS3 protein compared with chronic HCV subjects. Resolved subjects had a dose-dependent IL-22 response to recombinant NS3 compared with chronic HCV subjects. Conclusion: IL-22 production is associated with antigen-specific induction of CD4 + T cells in individuals that resolved HCV infection, suggesting a potential role for IL-22 in HCV clearance.

KW - IL-17

KW - IL-22

KW - T cell

KW - hepatitis C virus

KW - human PBMC

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In antigen-specific induction of IL-22 in human subjects that resolved HCV infection

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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