In situ electrochemical ELISA for specific identification of captured cancer cells

Tina Saberi Safaei, Reza M. Mohamadi, Edward H. Sargent, Shana O. Kelley*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

Circulating tumor cells (CTCs) are cancer cells disseminated from a tumor into the bloodstream. Their presence in patient blood samples has been associated with metastatic disease. Here, we report a simple system that enables the isolation and detection of these rare cancer cells. By developing a sensitive electrochemical ELISA method integrated within a microfluidic cell capture system, were we able to reliably detect very low levels of cancer cells in whole blood. Our results indicate that the new system provides the clinically relevant specificity and sensitivity needed for a convenient, point-of-need assay for cancer cell counting.

Original languageEnglish (US)
Pages (from-to)14165-14169
Number of pages5
JournalACS Applied Materials and Interfaces
Volume7
Issue number26
DOIs
StatePublished - Jul 8 2015
Externally publishedYes

Keywords

  • cancer cell detection
  • cancer cell isolation
  • electrochemistry
  • integrated chip
  • microfluidics

ASJC Scopus subject areas

  • Materials Science(all)

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