In situ electrochemical ELISA for specific identification of captured cancer cells

Tina Saberi Safaei; Reza M. Mohamadi; Edward H. Sargent; Shana O. Kelley

doi:10.1021/acsami.5b02404

In situ electrochemical ELISA for specific identification of captured cancer cells

Tina Saberi Safaei, Reza M. Mohamadi, Edward H. Sargent, Shana O. Kelley^*

^*Corresponding author for this work

Chemistry

Research output: Contribution to journal › Article › peer-review

54 Scopus citations

Abstract

Circulating tumor cells (CTCs) are cancer cells disseminated from a tumor into the bloodstream. Their presence in patient blood samples has been associated with metastatic disease. Here, we report a simple system that enables the isolation and detection of these rare cancer cells. By developing a sensitive electrochemical ELISA method integrated within a microfluidic cell capture system, were we able to reliably detect very low levels of cancer cells in whole blood. Our results indicate that the new system provides the clinically relevant specificity and sensitivity needed for a convenient, point-of-need assay for cancer cell counting.

Original language	English (US)
Pages (from-to)	14165-14169
Number of pages	5
Journal	ACS Applied Materials and Interfaces
Volume	7
Issue number	26
DOIs	https://doi.org/10.1021/acsami.5b02404
State	Published - Jul 8 2015

Keywords

cancer cell detection
cancer cell isolation
electrochemistry
integrated chip
microfluidics

ASJC Scopus subject areas

Materials Science(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1021/acsami.5b02404

Cite this

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title = "In situ electrochemical ELISA for specific identification of captured cancer cells",

abstract = "Circulating tumor cells (CTCs) are cancer cells disseminated from a tumor into the bloodstream. Their presence in patient blood samples has been associated with metastatic disease. Here, we report a simple system that enables the isolation and detection of these rare cancer cells. By developing a sensitive electrochemical ELISA method integrated within a microfluidic cell capture system, were we able to reliably detect very low levels of cancer cells in whole blood. Our results indicate that the new system provides the clinically relevant specificity and sensitivity needed for a convenient, point-of-need assay for cancer cell counting.",

keywords = "cancer cell detection, cancer cell isolation, electrochemistry, integrated chip, microfluidics",

author = "Safaei, {Tina Saberi} and Mohamadi, {Reza M.} and Sargent, {Edward H.} and Kelley, {Shana O.}",

note = "Publisher Copyright: {\textcopyright} 2015 American Chemical Society.",

year = "2015",

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doi = "10.1021/acsami.5b02404",

language = "English (US)",

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journal = "ACS Applied Materials and Interfaces",

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T1 - In situ electrochemical ELISA for specific identification of captured cancer cells

AU - Safaei, Tina Saberi

AU - Mohamadi, Reza M.

AU - Sargent, Edward H.

AU - Kelley, Shana O.

PY - 2015/7/8

Y1 - 2015/7/8

N2 - Circulating tumor cells (CTCs) are cancer cells disseminated from a tumor into the bloodstream. Their presence in patient blood samples has been associated with metastatic disease. Here, we report a simple system that enables the isolation and detection of these rare cancer cells. By developing a sensitive electrochemical ELISA method integrated within a microfluidic cell capture system, were we able to reliably detect very low levels of cancer cells in whole blood. Our results indicate that the new system provides the clinically relevant specificity and sensitivity needed for a convenient, point-of-need assay for cancer cell counting.

AB - Circulating tumor cells (CTCs) are cancer cells disseminated from a tumor into the bloodstream. Their presence in patient blood samples has been associated with metastatic disease. Here, we report a simple system that enables the isolation and detection of these rare cancer cells. By developing a sensitive electrochemical ELISA method integrated within a microfluidic cell capture system, were we able to reliably detect very low levels of cancer cells in whole blood. Our results indicate that the new system provides the clinically relevant specificity and sensitivity needed for a convenient, point-of-need assay for cancer cell counting.

KW - cancer cell detection

KW - cancer cell isolation

KW - electrochemistry

KW - integrated chip

KW - microfluidics

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DO - 10.1021/acsami.5b02404

M3 - Article

C2 - 25938818

AN - SCOPUS:84936883208

SN - 1944-8244

VL - 7

SP - 14165

EP - 14169

JO - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

IS - 26

ER -

In situ electrochemical ELISA for specific identification of captured cancer cells

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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