In situ end labelling of DNA to detect apoptotic cell death in a variety of human tumours

Suneel Mundle, Amna Iftikhar, Vilasini Shetty, Sairah Alvi, Sherry Dameron, Stephanie Gregory, Bridget Marcus, Seema Khan, Azra Raza*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

The present studies illustrate clinical applications of in situ end labelling (ISEL) of DNA to detect apoptosis in a variety of human malignancies including myelodysplastic syndromes (MDS, n=10), non-Hodgkin's lymphoma (NHL, n=10), head and neck cancer (n = 3), breast cancer (n = 1) and cervical cancer (n = 1). These studies also describe a new in situ double labelling technique to detect apoptosis and proliferation (S-phase cells) simultaneously in the same section of plastic embedded tissue. In vivo intravenous infusions of thymidine analogues (i.e. bromodeoxyuridine (BrdU) and/or iododeoxyuridine (IUdR)) followed by their detection with a specific monoclonal antibody in a plastic embedded biopsy, combined with ISEL in the same section, facilitated simultaneous estimations of apoptosis and proliferation. The most salient finding of these studies was excessive apoptosis in MDS including the cells in S-phase as indicated by uniquely double labelled cells in their bone marrow biopsies. On the other hand, a very low degree of apoptosis was observed in NHL and other solid tumours. Moreover, the solid tumours exhibited definite compartments of apoptosis and proliferation. Further experiments are underway to confirm these findings in a larger study in order to design appropriate therapeutic modalities for these disorders.

Original languageEnglish (US)
Pages (from-to)117-122
Number of pages6
JournalCell Death and Differentiation
Volume1
Issue number2
StatePublished - Dec 1 1994

Keywords

  • Apoptosis
  • In situ end labelling of DNA
  • Myelodysplastic Syndromes
  • Non-Hodgkin's Lymphoma
  • Proliferation

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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