In Situ Magnetic Control of Macroscale Nanoligand Density Regulates the Adhesion and Differentiation of Stem Cells

Chandra Khatua; Sunhong Min; Hee Joon Jung; Hee Joon Jung; Hee Joon Jung; Jeong Eun Shin; Na Li; Indong Jun; Hui Wen Liu; Gunhyu Bae; Hyojun Choi; Min Jun Ko; Yoo Sang Jeon; Yu Jin Kim; Joonbum Lee; Minji Ko; Gyubo Shim; Hongchul Shin; Sangbum Lee; Seok Chung; Young Keun Kim; Jae Jun Song; Vinayak P. Dravid; Vinayak P. Dravid; Vinayak P. Dravid; Heemin Kang

doi:10.1021/acs.nanolett.0c00559

In Situ Magnetic Control of Macroscale Nanoligand Density Regulates the Adhesion and Differentiation of Stem Cells

Chandra Khatua, Sunhong Min, Hee Joon Jung, Hee Joon Jung, Hee Joon Jung, Jeong Eun Shin, Na Li, Indong Jun, Hui Wen Liu, Gunhyu Bae, Hyojun Choi, Min Jun Ko, Yoo Sang Jeon, Yu Jin Kim, Joonbum Lee, Minji Ko, Gyubo Shim, Hongchul Shin, Sangbum Lee, Seok ChungYoung Keun Kim, Jae Jun Song, Vinayak P. Dravid, Vinayak P. Dravid, Vinayak P. Dravid, Heemin Kang^*

^*Corresponding author for this work

Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

Developing materials with remote controllability of macroscale ligand presentation can mimic extracellular matrix (ECM) remodeling to regulate cellular adhesion in vivo. Herein, we designed charged mobile nanoligands with superparamagnetic nanomaterials amine-functionalized and conjugated with polyethylene glycol linker and negatively charged RGD ligand. We coupled negatively a charged nanoligand to a positively charged substrate by optimizing electrostatic interactions to allow reversible planar movement. We demonstrate the imaging of both macroscale and in situ nanoscale nanoligand movement by magnetically attracting charged nanoligand to manipulate macroscale ligand density. We show that in situ magnetic control of attracting charged nanoligand facilitates stem cell adhesion, both in vitro and in vivo, with reversible control. Furthermore, we unravel that in situ magnetic attraction of charged nanoligand stimulates mechanosensing-mediated differentiation of stem cells. This remote controllability of ECM-mimicking reversible ligand variations is promising for regulating diverse reparative cellular processes in vivo.

Original language	English (US)
Pages (from-to)	4188-4196
Number of pages	9
Journal	Nano letters
Volume	20
Issue number	6
DOIs	https://doi.org/10.1021/acs.nanolett.0c00559
State	Published - Jun 10 2020

Funding

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (2019R1F1A1058720, 2019R1A2C3006587). This work was also supported by a Korea University Grant. This work was also conducted at the Korea Basic Science Institute. This work made use of the EPIC facility of Northwestern University’s NUANCE Center, which has received support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS-1542205), the MRSEC IRG2 program (NSF DMR-1720139) at the Materials Research Center, the International Institute for Nanotechnology (IIN), the Keck Foundation, and the State of Illinois through the IIN. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (2019R1F1A1058720, 2019R1A2C3006587). This work was also supported by a Korea University Grant. This work was also conducted at the Korea Basic Science Institute. This work made use of the EPIC facility of Northwestern University’s NUANCE Center, which has received support from the Soft and Hybrid Nanotechnology Experimental (SHyNE) Resource (NSF ECCS-1542205), the MRSEC IRG2 program (NSF DMR-1720139) at the Materials Research Center, the International Institute for Nanotechnology (IIN), the Keck Foundation, and the State of Illinois, through the IIN.

Keywords

cell adhesion
charged nanoligand
reversible ligand movement
spatial ligand movement
stem cell differentiation

ASJC Scopus subject areas

Bioengineering
General Chemistry
General Materials Science
Condensed Matter Physics
Mechanical Engineering

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10.1021/acs.nanolett.0c00559

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Khatua, C., Min, S., Jung, H. J., Jung, H. J., Jung, H. J., Shin, J. E., Li, N., Jun, I., Liu, H. W., Bae, G., Choi, H., Ko, M. J., Jeon, Y. S., Kim, Y. J., Lee, J., Ko, M., Shim, G., Shin, H., Lee, S., ... Kang, H. (2020). In Situ Magnetic Control of Macroscale Nanoligand Density Regulates the Adhesion and Differentiation of Stem Cells. Nano letters, 20(6), 4188-4196. https://doi.org/10.1021/acs.nanolett.0c00559

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title = "In Situ Magnetic Control of Macroscale Nanoligand Density Regulates the Adhesion and Differentiation of Stem Cells",

abstract = "Developing materials with remote controllability of macroscale ligand presentation can mimic extracellular matrix (ECM) remodeling to regulate cellular adhesion in vivo. Herein, we designed charged mobile nanoligands with superparamagnetic nanomaterials amine-functionalized and conjugated with polyethylene glycol linker and negatively charged RGD ligand. We coupled negatively a charged nanoligand to a positively charged substrate by optimizing electrostatic interactions to allow reversible planar movement. We demonstrate the imaging of both macroscale and in situ nanoscale nanoligand movement by magnetically attracting charged nanoligand to manipulate macroscale ligand density. We show that in situ magnetic control of attracting charged nanoligand facilitates stem cell adhesion, both in vitro and in vivo, with reversible control. Furthermore, we unravel that in situ magnetic attraction of charged nanoligand stimulates mechanosensing-mediated differentiation of stem cells. This remote controllability of ECM-mimicking reversible ligand variations is promising for regulating diverse reparative cellular processes in vivo.",

keywords = "cell adhesion, charged nanoligand, reversible ligand movement, spatial ligand movement, stem cell differentiation",

author = "Chandra Khatua and Sunhong Min and Jung, {Hee Joon} and Jung, {Hee Joon} and Jung, {Hee Joon} and Shin, {Jeong Eun} and Na Li and Indong Jun and Liu, {Hui Wen} and Gunhyu Bae and Hyojun Choi and Ko, {Min Jun} and Jeon, {Yoo Sang} and Kim, {Yu Jin} and Joonbum Lee and Minji Ko and Gyubo Shim and Hongchul Shin and Sangbum Lee and Seok Chung and Kim, {Young Keun} and Song, {Jae Jun} and Dravid, {Vinayak P.} and Dravid, {Vinayak P.} and Dravid, {Vinayak P.} and Heemin Kang",

note = "Publisher Copyright: Copyright {\textcopyright} 2020 American Chemical Society.",

year = "2020",

month = jun,

day = "10",

doi = "10.1021/acs.nanolett.0c00559",

language = "English (US)",

volume = "20",

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Khatua, C, Min, S, Jung, HJ, Jung, HJ, Jung, HJ, Shin, JE, Li, N, Jun, I, Liu, HW, Bae, G, Choi, H, Ko, MJ, Jeon, YS, Kim, YJ, Lee, J, Ko, M, Shim, G, Shin, H, Lee, S, Chung, S, Kim, YK, Song, JJ, Dravid, VP, Dravid, VP, Dravid, VP & Kang, H 2020, 'In Situ Magnetic Control of Macroscale Nanoligand Density Regulates the Adhesion and Differentiation of Stem Cells', Nano letters, vol. 20, no. 6, pp. 4188-4196. https://doi.org/10.1021/acs.nanolett.0c00559

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T1 - In Situ Magnetic Control of Macroscale Nanoligand Density Regulates the Adhesion and Differentiation of Stem Cells

AU - Khatua, Chandra

AU - Min, Sunhong

AU - Jung, Hee Joon

AU - Shin, Jeong Eun

AU - Li, Na

AU - Jun, Indong

AU - Liu, Hui Wen

AU - Bae, Gunhyu

AU - Choi, Hyojun

AU - Ko, Min Jun

AU - Jeon, Yoo Sang

AU - Kim, Yu Jin

AU - Lee, Joonbum

AU - Ko, Minji

AU - Shim, Gyubo

AU - Shin, Hongchul

AU - Lee, Sangbum

AU - Chung, Seok

AU - Kim, Young Keun

AU - Song, Jae Jun

AU - Dravid, Vinayak P.

AU - Kang, Heemin

PY - 2020/6/10

Y1 - 2020/6/10

N2 - Developing materials with remote controllability of macroscale ligand presentation can mimic extracellular matrix (ECM) remodeling to regulate cellular adhesion in vivo. Herein, we designed charged mobile nanoligands with superparamagnetic nanomaterials amine-functionalized and conjugated with polyethylene glycol linker and negatively charged RGD ligand. We coupled negatively a charged nanoligand to a positively charged substrate by optimizing electrostatic interactions to allow reversible planar movement. We demonstrate the imaging of both macroscale and in situ nanoscale nanoligand movement by magnetically attracting charged nanoligand to manipulate macroscale ligand density. We show that in situ magnetic control of attracting charged nanoligand facilitates stem cell adhesion, both in vitro and in vivo, with reversible control. Furthermore, we unravel that in situ magnetic attraction of charged nanoligand stimulates mechanosensing-mediated differentiation of stem cells. This remote controllability of ECM-mimicking reversible ligand variations is promising for regulating diverse reparative cellular processes in vivo.

AB - Developing materials with remote controllability of macroscale ligand presentation can mimic extracellular matrix (ECM) remodeling to regulate cellular adhesion in vivo. Herein, we designed charged mobile nanoligands with superparamagnetic nanomaterials amine-functionalized and conjugated with polyethylene glycol linker and negatively charged RGD ligand. We coupled negatively a charged nanoligand to a positively charged substrate by optimizing electrostatic interactions to allow reversible planar movement. We demonstrate the imaging of both macroscale and in situ nanoscale nanoligand movement by magnetically attracting charged nanoligand to manipulate macroscale ligand density. We show that in situ magnetic control of attracting charged nanoligand facilitates stem cell adhesion, both in vitro and in vivo, with reversible control. Furthermore, we unravel that in situ magnetic attraction of charged nanoligand stimulates mechanosensing-mediated differentiation of stem cells. This remote controllability of ECM-mimicking reversible ligand variations is promising for regulating diverse reparative cellular processes in vivo.

KW - cell adhesion

KW - charged nanoligand

KW - reversible ligand movement

KW - spatial ligand movement

KW - stem cell differentiation

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VL - 20

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JO - Nano letters

JF - Nano letters

IS - 6

ER -

In Situ Magnetic Control of Macroscale Nanoligand Density Regulates the Adhesion and Differentiation of Stem Cells

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