TY - JOUR
T1 - In utero tobacco smoke exposure, DNA methylation, and asthma in Latino children
AU - Neophytou, Andreas M.
AU - Oh, Sam S.
AU - Hu, Donglei
AU - Huntsman, Scott
AU - Eng, Celeste
AU - Rodríguez-Santana, José R.
AU - Kumar, Rajesh
AU - Balmes, John R.
AU - Eisen, Ellen A.
AU - Burchard, Esteban G.
N1 - Publisher Copyright:
© 2019 The Authors. Published by Wolters Kluwer Health, Inc.
PY - 2019/6/1
Y1 - 2019/6/1
N2 - Background: Maternal smoking during pregnancy is a risk factor for chronic disease later in life and has been associated with variability of DNA methylation at specific cytosine-phosphate-guanine (CpG) loci. We assessed the role of DNA methylation as a potential mediator of adverse effects of in utero tobacco smoke exposures on asthma outcomes in Latino children from the US mainland and Puerto Rico. Methods: Relationships between self-reported exposure and DNA methylation at CpG loci previously reported to be associated with maternal smoking were assessed in a subsample consisting of 572 children aged 8-21 years (310 cases with asthma, 262 healthy controls), sampled from a larger asthma case-control study. Subsequently, we assessed associations between top loci and asthma-related outcomes, followed by mediation analysis for loci for which associations with outcomes were observed. Results: Self-reported maternal smoking was associated with a -1.5% (95% confidence interval (CI) = -2.4%, -0.6%) lower methylation at CpG locus cg05575921 on the AHRR gene; a 1% increase in DNA methylation at the same locus resulted in an odds ratio (OR) of 0.90 (95% CI = 0.83, 0.96) for the odds of asthma. The OR for the indirect effect of maternal smoking on asthma mediated through methylation at the cg05575921 locus was 1.18 (95% CI = 1.07, 1.68), compared to the OR for the total effect of exposure in the parent study of 1.48 (95% CI = 1.03, 2.11). Conclusions: Our findings suggest potential mediation by DNA methylation in the association between maternal smoking during pregnancy and asthma status.
AB - Background: Maternal smoking during pregnancy is a risk factor for chronic disease later in life and has been associated with variability of DNA methylation at specific cytosine-phosphate-guanine (CpG) loci. We assessed the role of DNA methylation as a potential mediator of adverse effects of in utero tobacco smoke exposures on asthma outcomes in Latino children from the US mainland and Puerto Rico. Methods: Relationships between self-reported exposure and DNA methylation at CpG loci previously reported to be associated with maternal smoking were assessed in a subsample consisting of 572 children aged 8-21 years (310 cases with asthma, 262 healthy controls), sampled from a larger asthma case-control study. Subsequently, we assessed associations between top loci and asthma-related outcomes, followed by mediation analysis for loci for which associations with outcomes were observed. Results: Self-reported maternal smoking was associated with a -1.5% (95% confidence interval (CI) = -2.4%, -0.6%) lower methylation at CpG locus cg05575921 on the AHRR gene; a 1% increase in DNA methylation at the same locus resulted in an odds ratio (OR) of 0.90 (95% CI = 0.83, 0.96) for the odds of asthma. The OR for the indirect effect of maternal smoking on asthma mediated through methylation at the cg05575921 locus was 1.18 (95% CI = 1.07, 1.68), compared to the OR for the total effect of exposure in the parent study of 1.48 (95% CI = 1.03, 2.11). Conclusions: Our findings suggest potential mediation by DNA methylation in the association between maternal smoking during pregnancy and asthma status.
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U2 - 10.1097/EE9.0000000000000048
DO - 10.1097/EE9.0000000000000048
M3 - Article
C2 - 31342008
AN - SCOPUS:85084285382
SN - 2474-7882
VL - 3
JO - Environmental Epidemiology
JF - Environmental Epidemiology
IS - 3
M1 - e048
ER -
