In Vitro and In Vivo Approaches to Study Kinetochore-Microtubule Attachments During Mitosis

Sana Afreen, Amit Rahi, Adriana G. Landeros, Manas Chakraborty, Richard J. McKenney, Dileep Varma*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The separation of duplicated chromosomes during mitosis is a pivotal step in the process of cellular division. Therefore, the orchestrated events that take place to ensure proper attachment and stabilization of kMTs are keen areas of interest in the mitosis field. Here we describe the methods used to study kMT attachments via in vitro biochemical methods and in vivo cell biological approaches.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages123-138
Number of pages16
DOIs
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2415
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Cell cycle
  • Cell division
  • Cell synchronization
  • Chromosome
  • Kinetochores
  • Microscopy
  • Microtubules
  • Mitosis
  • Mitotic spindle
  • Photoactivation
  • TIR-FM
  • siRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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