Abstract
The separation of duplicated chromosomes during mitosis is a pivotal step in the process of cellular division. Therefore, the orchestrated events that take place to ensure proper attachment and stabilization of kMTs are keen areas of interest in the mitosis field. Here we describe the methods used to study kMT attachments via in vitro biochemical methods and in vivo cell biological approaches.
Original language | English (US) |
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Title of host publication | Methods in Molecular Biology |
Publisher | Humana Press Inc. |
Pages | 123-138 |
Number of pages | 16 |
DOIs | |
State | Published - 2022 |
Publication series
Name | Methods in Molecular Biology |
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Volume | 2415 |
ISSN (Print) | 1064-3745 |
ISSN (Electronic) | 1940-6029 |
Funding
We would like to acknowledge the National Institute of Health (NIH) Grant R01GM135391 to D.V. from the National Institute of General Medical Sciences (NIGMS). Sana Afreen and Amit Rahi contributed equally to this work.
Keywords
- Cell cycle
- Cell division
- Cell synchronization
- Chromosome
- Kinetochores
- Microscopy
- Microtubules
- Mitosis
- Mitotic spindle
- Photoactivation
- TIR-FM
- siRNA
ASJC Scopus subject areas
- Genetics
- Molecular Biology