In Vitro and In Vivo Approaches to Study Kinetochore-Microtubule Attachments During Mitosis

Sana Afreen; Amit Rahi; Adriana G. Landeros; Manas Chakraborty; Richard J. McKenney; Dileep Varma

doi:10.1007/978-1-0716-1904-9_9

In Vitro and In Vivo Approaches to Study Kinetochore-Microtubule Attachments During Mitosis

Sana Afreen, Amit Rahi, Adriana G. Landeros, Manas Chakraborty, Richard J. McKenney, Dileep Varma^*

^*Corresponding author for this work

Cell & Developmental Biology

Research output: Chapter in Book/Report/Conference proceeding › Chapter

3 Scopus citations

Abstract

The separation of duplicated chromosomes during mitosis is a pivotal step in the process of cellular division. Therefore, the orchestrated events that take place to ensure proper attachment and stabilization of kMTs are keen areas of interest in the mitosis field. Here we describe the methods used to study kMT attachments via in vitro biochemical methods and in vivo cell biological approaches.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	123-138
Number of pages	16
DOIs	https://doi.org/10.1007/978-1-0716-1904-9_9
State	Published - 2022

Publication series

Name	Methods in Molecular Biology
Volume	2415
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Funding

We would like to acknowledge the National Institute of Health (NIH) Grant R01GM135391 to D.V. from the National Institute of General Medical Sciences (NIGMS). Sana Afreen and Amit Rahi contributed equally to this work.

Keywords

Cell cycle
Cell division
Cell synchronization
Chromosome
Kinetochores
Microscopy
Microtubules
Mitosis
Mitotic spindle
Photoactivation
TIR-FM
siRNA

ASJC Scopus subject areas

Genetics
Molecular Biology

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10.1007/978-1-0716-1904-9_9

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abstract = "The separation of duplicated chromosomes during mitosis is a pivotal step in the process of cellular division. Therefore, the orchestrated events that take place to ensure proper attachment and stabilization of kMTs are keen areas of interest in the mitosis field. Here we describe the methods used to study kMT attachments via in vitro biochemical methods and in vivo cell biological approaches.",

keywords = "Cell cycle, Cell division, Cell synchronization, Chromosome, Kinetochores, Microscopy, Microtubules, Mitosis, Mitotic spindle, Photoactivation, TIR-FM, siRNA",

author = "Sana Afreen and Amit Rahi and Landeros, {Adriana G.} and Manas Chakraborty and McKenney, {Richard J.} and Dileep Varma",

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AU - Afreen, Sana

AU - Rahi, Amit

AU - Landeros, Adriana G.

AU - Chakraborty, Manas

AU - McKenney, Richard J.

AU - Varma, Dileep

PY - 2022

Y1 - 2022

N2 - The separation of duplicated chromosomes during mitosis is a pivotal step in the process of cellular division. Therefore, the orchestrated events that take place to ensure proper attachment and stabilization of kMTs are keen areas of interest in the mitosis field. Here we describe the methods used to study kMT attachments via in vitro biochemical methods and in vivo cell biological approaches.

AB - The separation of duplicated chromosomes during mitosis is a pivotal step in the process of cellular division. Therefore, the orchestrated events that take place to ensure proper attachment and stabilization of kMTs are keen areas of interest in the mitosis field. Here we describe the methods used to study kMT attachments via in vitro biochemical methods and in vivo cell biological approaches.

KW - Cell cycle

KW - Cell division

KW - Cell synchronization

KW - Chromosome

KW - Kinetochores

KW - Microscopy

KW - Microtubules

KW - Mitosis

KW - Mitotic spindle

KW - Photoactivation

KW - TIR-FM

KW - siRNA

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T3 - Methods in Molecular Biology

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BT - Methods in Molecular Biology

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ER -

In Vitro and In Vivo Approaches to Study Kinetochore-Microtubule Attachments During Mitosis

Abstract

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Keywords

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