In vitro and preliminary in vivo validation of echo particle image velocimetry in carotid vascular imaging

Fuxing Zhang, Craig Lanning, Luciano Mazzaro, Alex J. Barker, Phillip E. Gates, W. David Strain, Jonathan Fulford, Oliver E. Gosling, Angela C. Shore, Nick G. Bellenger, Bryan Rech, Jiusheng Chen, James Chen, Robin Shandas*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

75 Scopus citations


Noninvasive, easy-to-use and accurate measurements of wall shear stress (WSS) in human blood vessels have always been challenging in clinical applications. Echo particle image velocimetry (Echo PIV) has shown promise for clinical measurements of local hemodynamics and wall shear rate. Thus far, however, the method has only been validated under simple flow conditions. In this study, we validated Echo PIV under in vitro and in vivo conditions. For in vitro validation, we used an anatomically correct, compliant carotid bifurcation flow phantom with pulsatile flow conditions, using optical particle image velocimetry (optical PIV) as the reference standard. For in vivo validation, we compared Echo PIV-derived 2-D velocity fields obtained at the carotid bifurcation in five normal subjects against phase-contrast magnetic resonance imaging (PC-MRI)-derived velocity measurements obtained at the same locations. For both studies, time-dependent, 2-D, two-component velocity vectors; peak/centerline velocity, flow rate and wall shear rate (WSR) waveforms at the common carotid artery (CCA), carotid bifurcation and distal internal carotid artery (ICA) were examined. Linear regression, correlation analysis and Bland-Altman analysis were used to quantify the agreement of different waveforms measured by the two techniques. In vitro results showed that Echo PIV produced good images of time-dependent velocity vector maps over the cardiac cycle with excellent temporal (up to 0.7 ms) and spatial (∼0.5 mm) resolutions and quality, comparable with optical PIV results. Further, good agreement was found between Echo PIV and optical PIV results for velocity and WSR measurements. In vivo results also showed good agreement between Echo PIV velocities and phase contrast MRI velocities. We conclude that Echo PIV provides accurate velocity vector and WSR measurements in the carotid bifurcation and has significant potential as a clinical tool for cardiovascular hemodynamics evaluation.

Original languageEnglish (US)
Pages (from-to)450-464
Number of pages15
JournalUltrasound in Medicine and Biology
Issue number3
StatePublished - Mar 2011


  • Atherosclerosis
  • Blood velocity vector
  • Echo PIV
  • Human carotid artery
  • Ultrasound contrast agents
  • Validation
  • Wall shear rate
  • Wall shear stress

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Biophysics
  • Acoustics and Ultrasonics


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