Abstract
Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. Objectives: The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Material and Methods: Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (gHAp.cm−3), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Results: Fluoride gel presented higher %SHR followed by ACP 3 min (p<0.001). No difference (p = 0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for %SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. Conclusion: In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 596-603 |
| Number of pages | 8 |
| Journal | Journal of Applied Oral Science |
| Volume | 25 |
| Issue number | 6 |
| DOIs | |
| State | Published - Nov 1 2017 |
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Keywords
- Calcium phosphates
- Dental enamel
- Fluoride
- Tooth remineralization
- X-ray microtomography
ASJC Scopus subject areas
- Dentistry(all)
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In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization. / Vieira, Ana Elisa De Mello; Danelon, Marcelle; Da Camara, Danielle Mendes; Rosselli, Eliana Rodrigues; Stock, Stuart R; Cannon, Mark L.; Xiao, Xianghui; De Carlo, Francesco; Delbem, Alberto Carlos Botazzo.
In: Journal of Applied Oral Science, Vol. 25, No. 6, 01.11.2017, p. 596-603.Research output: Contribution to journal › Article
TY - JOUR
T1 - In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization
AU - Vieira, Ana Elisa De Mello
AU - Danelon, Marcelle
AU - Da Camara, Danielle Mendes
AU - Rosselli, Eliana Rodrigues
AU - Stock, Stuart R
AU - Cannon, Mark L.
AU - Xiao, Xianghui
AU - De Carlo, Francesco
AU - Delbem, Alberto Carlos Botazzo
PY - 2017/11/1
Y1 - 2017/11/1
N2 - Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. Objectives: The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Material and Methods: Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (gHAp.cm−3), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Results: Fluoride gel presented higher %SHR followed by ACP 3 min (p<0.001). No difference (p = 0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for %SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. Conclusion: In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.
AB - Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. Objectives: The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Material and Methods: Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (gHAp.cm−3), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Results: Fluoride gel presented higher %SHR followed by ACP 3 min (p<0.001). No difference (p = 0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for %SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. Conclusion: In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.
KW - Calcium phosphates
KW - Dental enamel
KW - Fluoride
KW - Tooth remineralization
KW - X-ray microtomography
UR - http://www.scopus.com/inward/record.url?scp=85036637982&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85036637982&partnerID=8YFLogxK
U2 - 10.1590/1678-7757-2016-0513
DO - 10.1590/1678-7757-2016-0513
M3 - Article
VL - 25
SP - 596
EP - 603
JO - Journal of Applied Oral Science
JF - Journal of Applied Oral Science
SN - 1678-7757
IS - 6
ER -