In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization

Ana Elisa De Mello Vieira, Marcelle Danelon, Danielle Mendes Da Camara, Eliana Rodrigues Rosselli, Stuart R. Stock, Mark L. Cannon, Xianghui Xiao, Francesco De Carlo, Alberto Carlos Botazzo Delbem*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. Objectives: The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Material and Methods: Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (−3), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Results: Fluoride gel presented higher %SHR followed by ACP 3 min (p<0.001). No difference (p = 0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for %SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. Conclusion: In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.

Original languageEnglish (US)
Pages (from-to)596-603
Number of pages8
JournalJournal of Applied Oral Science
Issue number6
StatePublished - Nov 1 2017


  • Calcium phosphates
  • Dental enamel
  • Fluoride
  • Tooth remineralization
  • X-ray microtomography

ASJC Scopus subject areas

  • Dentistry(all)


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