In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization

Ana Elisa De Mello Vieira, Marcelle Danelon, Danielle Mendes Da Camara, Eliana Rodrigues Rosselli, Stuart R Stock, Mark L. Cannon, Xianghui Xiao, Francesco De Carlo, Alberto Carlos Botazzo Delbem*

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. Objectives: The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Material and Methods: Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (gHAp.cm−3), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Results: Fluoride gel presented higher %SHR followed by ACP 3 min (p<0.001). No difference (p = 0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for %SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. Conclusion: In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.

Original languageEnglish (US)
Pages (from-to)596-603
Number of pages8
JournalJournal of Applied Oral Science
Volume25
Issue number6
DOIs
StatePublished - Nov 1 2017

Fingerprint

Phosphopeptides
Dental Enamel
Ointments
Caseins
Hardness
Gels
Fluorides
Minerals
Placebos
Synchrotrons
amorphous calcium phosphate
In Vitro Techniques
Analysis of Variance
Tooth
Tomography
calcium phosphate
Students

Keywords

  • Calcium phosphates
  • Dental enamel
  • Fluoride
  • Tooth remineralization
  • X-ray microtomography

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Vieira, A. E. D. M., Danelon, M., Da Camara, D. M., Rosselli, E. R., Stock, S. R., Cannon, M. L., ... Delbem, A. C. B. (2017). In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization. Journal of Applied Oral Science, 25(6), 596-603. https://doi.org/10.1590/1678-7757-2016-0513
Vieira, Ana Elisa De Mello ; Danelon, Marcelle ; Da Camara, Danielle Mendes ; Rosselli, Eliana Rodrigues ; Stock, Stuart R ; Cannon, Mark L. ; Xiao, Xianghui ; De Carlo, Francesco ; Delbem, Alberto Carlos Botazzo. / In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization. In: Journal of Applied Oral Science. 2017 ; Vol. 25, No. 6. pp. 596-603.
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title = "In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization",
abstract = "Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. Objectives: The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Material and Methods: Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2{\%} NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery ({\%}SHR) and to calculate mineral concentration (gHAp.cm−3), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Results: Fluoride gel presented higher {\%}SHR followed by ACP 3 min (p<0.001). No difference (p = 0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for {\%}SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. Conclusion: In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.",
keywords = "Calcium phosphates, Dental enamel, Fluoride, Tooth remineralization, X-ray microtomography",
author = "Vieira, {Ana Elisa De Mello} and Marcelle Danelon and {Da Camara}, {Danielle Mendes} and Rosselli, {Eliana Rodrigues} and Stock, {Stuart R} and Cannon, {Mark L.} and Xianghui Xiao and {De Carlo}, Francesco and Delbem, {Alberto Carlos Botazzo}",
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Vieira, AEDM, Danelon, M, Da Camara, DM, Rosselli, ER, Stock, SR, Cannon, ML, Xiao, X, De Carlo, F & Delbem, ACB 2017, 'In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization', Journal of Applied Oral Science, vol. 25, no. 6, pp. 596-603. https://doi.org/10.1590/1678-7757-2016-0513

In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization. / Vieira, Ana Elisa De Mello; Danelon, Marcelle; Da Camara, Danielle Mendes; Rosselli, Eliana Rodrigues; Stock, Stuart R; Cannon, Mark L.; Xiao, Xianghui; De Carlo, Francesco; Delbem, Alberto Carlos Botazzo.

In: Journal of Applied Oral Science, Vol. 25, No. 6, 01.11.2017, p. 596-603.

Research output: Contribution to journalArticle

TY - JOUR

T1 - In vitro effect of amorphous calcium phosphate paste applied for extended periods of time on enamel remineralization

AU - Vieira, Ana Elisa De Mello

AU - Danelon, Marcelle

AU - Da Camara, Danielle Mendes

AU - Rosselli, Eliana Rodrigues

AU - Stock, Stuart R

AU - Cannon, Mark L.

AU - Xiao, Xianghui

AU - De Carlo, Francesco

AU - Delbem, Alberto Carlos Botazzo

PY - 2017/11/1

Y1 - 2017/11/1

N2 - Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. Objectives: The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Material and Methods: Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (gHAp.cm−3), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Results: Fluoride gel presented higher %SHR followed by ACP 3 min (p<0.001). No difference (p = 0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for %SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. Conclusion: In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.

AB - Dental applications based on the unique characteristics of amorphous calcium phosphate stabilized by casein phosphopeptides (CPP-ACP) have been proposed, as well as the improvement of its properties. Objectives: The objective of this study was to determine the ability of topically applied CPP-ACP from a commercial product to remineralize subsurface lesions when applied for extended periods of time (3 h and 8 h). Material and Methods: Artificially induced carious lesions were produced in 50 bovine enamel blocks previously selected by surface hardness. After treatments with gel without F and CPP-ACP applied for 1 minute (Placebo); 2% NaF neutral gel applied for 1 minute (Fluoride 1 min); CPP-ACP applied for 3 min (ACP 3 min); and CPP-ACP applied for 3 h (ACP 3 h) and for 8 h (ACP 8 h), the enamel blocks were submitted to the remineralization pH-cycling. Surface hardness and synchrotron micro-tomography were used to determine the percentage of surface hardness recovery (%SHR) and to calculate mineral concentration (gHAp.cm−3), respectively. The data were submitted to ANOVA followed by the Student-Newman-Keuls test (p<0.05). Results: Fluoride gel presented higher %SHR followed by ACP 3 min (p<0.001). No difference (p = 0.148) was found for Placebo, ACP 3 h and ACP 8 h groups for %SHR. Fluoride gel showed greater mineral concentration (p<0.001) when compared with the other groups. ACP 3 min demonstrated a significant difference (p<0.001) from ACP 3 h and ACP 8 h. The ACP 3 h and 8 h presented a subsurface lesion with development of laminations in all blocks. Conclusion: In this in vitro study the use of CPP-ACP for extended periods of time did not produce an additive effect in the remineralization process.

KW - Calcium phosphates

KW - Dental enamel

KW - Fluoride

KW - Tooth remineralization

KW - X-ray microtomography

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