In vitro evolution of high-titer, virus-like vesicles containing a single structural protein

Nina F. Rose, Linda Buonocore, John B. Schell, Anasuya Chattopadhyay, Kapil Bahl, Xinran Liu, John K. Rose*, Robert A Lamb

*Corresponding author for this work

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Self-propagating, infectious, virus-like vesicles (VLVs) are generated when an alphavirus RNA replicon expresses the vesicular stomatitis virus glycoprotein (VSV G) as the only structural protein. The mechanism that generates these VLVs lacking a capsid protein has remained a mystery for over 20 years. We present evidence that VLVs arise from membrane-enveloped RNA replication factories (spherules) containing VSV G protein that are largely trapped on the cell surface. After extensive passaging, VLVs evolve to grow to high titers through acquisition of multiple point mutations in their nonstructural replicase proteins. We reconstituted these mutations into a plasmid-based system from which hightiter VLVs can be recovered. One of these mutations generates a late domain motif (PTAP) that is critical for high-titer VLV production. We propose a model in which the VLVs have evolved in vitro to exploit a cellular budding pathway that is hijacked by many enveloped viruses, allowing them to bud efficiently from the cell surface. Our results suggest a basic mechanism of propagation that may have been used by primitive RNA viruses lacking capsid proteins. Capsids may have evolved later to allow more efficient packaging of RNA, greater virus stability, and evasion of innate immunity.

Original languageEnglish (US)
Pages (from-to)16866-16871
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number47
DOIs
StatePublished - Nov 25 2014

Keywords

  • Evolution
  • Late domain
  • SFV replicon
  • VSV glycoprotein

ASJC Scopus subject areas

  • General

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