In Vitro Model of the Epidermis: Connecting Protein Function to 3D Structure

Christopher Arnette; Jennifer L. Koetsier; Paul Hoover; Spiro Getsios; Kathleen J. Green

doi:10.1016/bs.mie.2015.07.015

In Vitro Model of the Epidermis: Connecting Protein Function to 3D Structure

Christopher Arnette, Jennifer L. Koetsier, Paul Hoover, Spiro Getsios, Kathleen J. Green^*

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter

49 Scopus citations

Abstract

Much of our understanding of the biological processes that underlie cellular functions in humans, such as cell-cell communication, intracellular signaling, and transcriptional and posttranscriptional control of gene expression, has been acquired from studying cells in a two-dimensional (2D) tissue culture environment. However, it has become increasingly evident that the 2D environment does not support certain cell functions. The need for more physiologically relevant models prompted the development of three-dimensional (3D) cultures of epithelial, endothelial, and neuronal tissues (Shamir & Ewald, 2014). These models afford investigators with powerful tools to study the contribution of spatial organization, often in the context of relevant extracellular matrix and stromal components, to cellular and tissue homeostasis in normal and disease states.

Original language	English (US)
Title of host publication	Methods in Enzymology
Publisher	Academic Press Inc
Pages	287-308
Number of pages	22
DOIs	https://doi.org/10.1016/bs.mie.2015.07.015
State	Published - 2016

Publication series

Name	Methods in Enzymology
Volume	569
ISSN (Print)	0076-6879
ISSN (Electronic)	1557-7988

Funding

This work was supported in part by grants to K.J.G. from the National Institute of Arthritis and Musculoskeletal and Skin Diseases (R37 AR043380 and RO1 AR041836), the National Cancer Institute (R01 CA122151), the Joseph L. Mayberry Endowment, and in part by a grant to S.G. from the National Institute of Arthritis and Musculoskeletal and Skin Diseases (RO1 AR062110) and the Northwestern University Skin Disease Research Core (P30 AR057216).

Keywords

Desmoglein
Epidermis
Keratinocyte
Organotypic

ASJC Scopus subject areas

Molecular Biology
Biochemistry

Access to Document

10.1016/bs.mie.2015.07.015

Cite this

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abstract = "Much of our understanding of the biological processes that underlie cellular functions in humans, such as cell-cell communication, intracellular signaling, and transcriptional and posttranscriptional control of gene expression, has been acquired from studying cells in a two-dimensional (2D) tissue culture environment. However, it has become increasingly evident that the 2D environment does not support certain cell functions. The need for more physiologically relevant models prompted the development of three-dimensional (3D) cultures of epithelial, endothelial, and neuronal tissues (Shamir & Ewald, 2014). These models afford investigators with powerful tools to study the contribution of spatial organization, often in the context of relevant extracellular matrix and stromal components, to cellular and tissue homeostasis in normal and disease states.",

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PY - 2016

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AB - Much of our understanding of the biological processes that underlie cellular functions in humans, such as cell-cell communication, intracellular signaling, and transcriptional and posttranscriptional control of gene expression, has been acquired from studying cells in a two-dimensional (2D) tissue culture environment. However, it has become increasingly evident that the 2D environment does not support certain cell functions. The need for more physiologically relevant models prompted the development of three-dimensional (3D) cultures of epithelial, endothelial, and neuronal tissues (Shamir & Ewald, 2014). These models afford investigators with powerful tools to study the contribution of spatial organization, often in the context of relevant extracellular matrix and stromal components, to cellular and tissue homeostasis in normal and disease states.

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KW - Keratinocyte

KW - Organotypic

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In Vitro Model of the Epidermis: Connecting Protein Function to 3D Structure

Abstract

Publication series

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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