TY - CHAP
T1 - In Vitro Model of the Epidermis
T2 - Connecting Protein Function to 3D Structure
AU - Arnette, Christopher
AU - Koetsier, Jennifer L.
AU - Hoover, Paul
AU - Getsios, Spiro
AU - Green, Kathleen J.
N1 - Funding Information:
This work was supported in part by grants to K.J.G. from the National Institute of Arthritis and Musculoskeletal and Skin Diseases (R37 AR043380 and RO1 AR041836), the National Cancer Institute (R01 CA122151), the Joseph L. Mayberry Endowment, and in part by a grant to S.G. from the National Institute of Arthritis and Musculoskeletal and Skin Diseases (RO1 AR062110) and the Northwestern University Skin Disease Research Core (P30 AR057216).
PY - 2016
Y1 - 2016
N2 - Much of our understanding of the biological processes that underlie cellular functions in humans, such as cell-cell communication, intracellular signaling, and transcriptional and posttranscriptional control of gene expression, has been acquired from studying cells in a two-dimensional (2D) tissue culture environment. However, it has become increasingly evident that the 2D environment does not support certain cell functions. The need for more physiologically relevant models prompted the development of three-dimensional (3D) cultures of epithelial, endothelial, and neuronal tissues (Shamir & Ewald, 2014). These models afford investigators with powerful tools to study the contribution of spatial organization, often in the context of relevant extracellular matrix and stromal components, to cellular and tissue homeostasis in normal and disease states.
AB - Much of our understanding of the biological processes that underlie cellular functions in humans, such as cell-cell communication, intracellular signaling, and transcriptional and posttranscriptional control of gene expression, has been acquired from studying cells in a two-dimensional (2D) tissue culture environment. However, it has become increasingly evident that the 2D environment does not support certain cell functions. The need for more physiologically relevant models prompted the development of three-dimensional (3D) cultures of epithelial, endothelial, and neuronal tissues (Shamir & Ewald, 2014). These models afford investigators with powerful tools to study the contribution of spatial organization, often in the context of relevant extracellular matrix and stromal components, to cellular and tissue homeostasis in normal and disease states.
KW - Desmoglein
KW - Epidermis
KW - Keratinocyte
KW - Organotypic
UR - http://www.scopus.com/inward/record.url?scp=84954361018&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84954361018&partnerID=8YFLogxK
U2 - 10.1016/bs.mie.2015.07.015
DO - 10.1016/bs.mie.2015.07.015
M3 - Chapter
C2 - 26778564
AN - SCOPUS:84954361018
T3 - Methods in Enzymology
SP - 287
EP - 308
BT - Methods in Enzymology
PB - Academic Press Inc
ER -