In Vitro Model of the Epidermis: Connecting Protein Function to 3D Structure

Christopher Arnette, Jennifer L. Koetsier, Paul Hoover, Spiro Getsios, Kathleen J. Green*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

43 Scopus citations


Much of our understanding of the biological processes that underlie cellular functions in humans, such as cell-cell communication, intracellular signaling, and transcriptional and posttranscriptional control of gene expression, has been acquired from studying cells in a two-dimensional (2D) tissue culture environment. However, it has become increasingly evident that the 2D environment does not support certain cell functions. The need for more physiologically relevant models prompted the development of three-dimensional (3D) cultures of epithelial, endothelial, and neuronal tissues (Shamir & Ewald, 2014). These models afford investigators with powerful tools to study the contribution of spatial organization, often in the context of relevant extracellular matrix and stromal components, to cellular and tissue homeostasis in normal and disease states.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc
Number of pages22
StatePublished - 2016

Publication series

NameMethods in Enzymology
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988


  • Desmoglein
  • Epidermis
  • Keratinocyte
  • Organotypic

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry


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