In vitro production of an anti-DNA idiotype by lymphocytes of normal subjects and patients with systemic lupus erythematosus

Syamal K. Datta*, Yaakov Naparstek, Robert S. Schwartz

*Corresponding author for this work

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

We investigated whether normal B cells can synthesize antibodies with an idiotypic marker that occurs with high frequency in anti-DNA antibodies of patients with systemic lupus erythematosus (SLE). This idiotype, Id 16 6, has been found in the serum of patients with active SLE and in monoclonal anti-DNA antibodies derived from unrelated patients with the disease. We found that cultured lymphocytes of all normal subjects tested produced Id 16 6 when stimulated by pokeweed mitogen (PWM). By contrast, lymphocytes from SLE patients produced Id 16 6 even without mitogenic stimulation, whether or not they were obtained from patients in remission or relapse. Relapsed patients' lymphocytes spontaneously produced the highest levels of Id 16 6 which was found in IgG and IgA, in addition to IgM. The majority of Id 16 6 produced by PWM-stimulated lymphocytes from either normal subjects or patients in remission did not bind to nucleic acid. In relapse, however, the nucleic acid-binding proportion of Id 16 6 rose substantially, indicating that the spontaneously activated B cells in active SLE differ from the subset of B cells that produce Id 16 6 upon PWM stimulation. The findings suggest that the lupus Id 16 6 family is conserved in normal individuals and it consists of two populations of antibodies with different antigenic specificities. The major set is not directed against nucleic acid antigens; its antigenic specificity is unknown and it dominates the Id 16 6 family that appears after PWM stimulation. The other, minor set binds to nucleic acids and becomes prominent in clinically active lupus. These two sets of idiotypically related antibodies may be connected by an immunoregulatory network.

Original languageEnglish (US)
Pages (from-to)302-318
Number of pages17
JournalClinical Immunology and Immunopathology
Volume38
Issue number3
DOIs
StatePublished - Mar 1986

ASJC Scopus subject areas

  • Immunology and Allergy
  • Pathology and Forensic Medicine
  • Immunology

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