In vitro production of megakaryocytes from PIXY321 versus GM-CSF- mobilized peripheral blood progenitor cells

P. Lefebvre, J. N. Winter, A. W. Rademaker, C. Goolsby, I. Cohen*

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

The generation of megakaryocytes (MK) from cultured peripheral blood progenitor cells (PBSC), harvested via apheresis, from 18 female breast cancer patients treated with either PIXY321 or GM-CSF was compared. Nonadherent mononuclear cells (MNC) were cultured in liquid suspension with 50 U/ml thrombopoietin (TPO) and 2.5% autologous heparinized plasma for 12 days. Flow cytometric analysis was used to measure the percentage of CD34+ on day 1 and CD41+ cells on day 12. The frequency of CD34+ cells was greater in GM-CSF-mobilized samples than in PIXY321-mobilized samples, and MK/MNC yields correlated directly with the number of CD34+ cells seeded. PIXY321-mobilized samples produced more MKs per CD34+ cell than GM-CSF- mobilized samples. Overall, there was no significant difference in the MK/MNC yield between PIXY321- and GM-CSF-mobilized samples. Cyclophosphamide (CY) increased the frequency of CD34+ cells and the corresponding MK/MNC yield for both cytokines, but had no effect on the MK/CD34+ yield. Compared to GM- CSF, PIXY321 mobilization resulted in increased CD34+ cell commitment to the MK lineage.

Original languageEnglish (US)
Pages (from-to)112-118
Number of pages7
JournalStem Cells
Volume15
Issue number2
DOIs
StatePublished - Jan 1 1997

Keywords

  • Cyclophosphamide
  • GM- CSF
  • Megakaryocytes
  • PIXY321
  • Peripheral blood progenitor cells
  • Thrombopoietin

ASJC Scopus subject areas

  • Molecular Medicine
  • Developmental Biology
  • Cell Biology

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