In vitro reassembly of squid brain intermediate filaments (neurofilaments): Purification by assembly-disassembly

Robert V. Zackroff, Robert D. Goldman

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Intermediate filaments from squid brain tissue were reassembled in vitro and purified by two cycles of assembly and disassembly. Purified squid brain filaments contained one major polypeptide (60,000 daltons), which constituted about 70 percent of the total protein, and three minor polypeptides (74,000, 100,000, and 220,000 daltons). Squid brain intermediate filaments were reconstituted from rod-shaped protofilamentous subunits. In addition to the intermediate filaments, dense bodies which may function in intermediate filament nucleation or organization were retained through two purification cycles.

Original languageEnglish (US)
Pages (from-to)1152-1155
Number of pages4
JournalScience
Volume208
Issue number4448
DOIs
StatePublished - 1980

ASJC Scopus subject areas

  • General

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