TY - JOUR
T1 - In vitro reassembly of squid brain intermediate filaments (neurofilaments)
T2 - Purification by assembly-disassembly
AU - Zackroff, Robert V.
AU - Goldman, Robert D.
PY - 1980
Y1 - 1980
N2 - Intermediate filaments from squid brain tissue were reassembled in vitro and purified by two cycles of assembly and disassembly. Purified squid brain filaments contained one major polypeptide (60,000 daltons), which constituted about 70 percent of the total protein, and three minor polypeptides (74,000, 100,000, and 220,000 daltons). Squid brain intermediate filaments were reconstituted from rod-shaped protofilamentous subunits. In addition to the intermediate filaments, dense bodies which may function in intermediate filament nucleation or organization were retained through two purification cycles.
AB - Intermediate filaments from squid brain tissue were reassembled in vitro and purified by two cycles of assembly and disassembly. Purified squid brain filaments contained one major polypeptide (60,000 daltons), which constituted about 70 percent of the total protein, and three minor polypeptides (74,000, 100,000, and 220,000 daltons). Squid brain intermediate filaments were reconstituted from rod-shaped protofilamentous subunits. In addition to the intermediate filaments, dense bodies which may function in intermediate filament nucleation or organization were retained through two purification cycles.
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U2 - 10.1126/science.7189605
DO - 10.1126/science.7189605
M3 - Article
C2 - 7189605
AN - SCOPUS:0019151856
SN - 0036-8075
VL - 208
SP - 1152
EP - 1155
JO - Science
JF - Science
IS - 4448
ER -