TY - JOUR
T1 - In vitro tissue-digesting properties of krill enzymes compared with Fibrinolysin/DNAse, papain and placebo
AU - Mekkes, J. R.
AU - Le Poole, I. C.
AU - Das, P. K.
AU - Kammeyer, A.
AU - Westerhof, W.
PY - 1997/4
Y1 - 1997/4
N2 - Wound debridement, tile removal of necrotic tissue, can be achieved with proteolytic enzymes. Recently, a new multi-enzyme preparation, krill enzyme, isolated from Antarctic shrimp-like organisms (Euphausia superba), was reported to possess powerful proteolytic activity towards protein substrates. In this paper, we study the in vitro digestive properties of krill enzymes towards whole tissue, compared with placebo, papain, and fibrinolysin/DNAse. Freshly obtained skin specimens were exposed for 3 days to krill enzymes (3; 0.6 and 0.06 U/ml), papain (120; 60; 6 and 0.6 U/ml), fibrinolysin/DNAse (2.5/1500 E and 1/600 E), and phosphate-buffered saline control solution. Tissue digestion was estimated by measuring wet wt, dry wt, and histological examination. After 72 hr of exposure to 3 U/ml krill enzymes, the dry wt of the specimens was reduced to 2.7% ± 1.9 (SEM, n = 5), compared with 31.0% ± 2.7 for placebo, 25.7% ± 2.5 for 120 U/ml papain, and 24.5% ± 3.3 for 2.5/1500 E/ml fibrinolysin/DNAse. The differences between krill enzymes and fibrinolysin/DNAse, papain, and control solution were statistically significant (p < 0.007). These data suggest that krill enzymes are more active than other commonly available proteolytic agents used for wound debridement.
AB - Wound debridement, tile removal of necrotic tissue, can be achieved with proteolytic enzymes. Recently, a new multi-enzyme preparation, krill enzyme, isolated from Antarctic shrimp-like organisms (Euphausia superba), was reported to possess powerful proteolytic activity towards protein substrates. In this paper, we study the in vitro digestive properties of krill enzymes towards whole tissue, compared with placebo, papain, and fibrinolysin/DNAse. Freshly obtained skin specimens were exposed for 3 days to krill enzymes (3; 0.6 and 0.06 U/ml), papain (120; 60; 6 and 0.6 U/ml), fibrinolysin/DNAse (2.5/1500 E and 1/600 E), and phosphate-buffered saline control solution. Tissue digestion was estimated by measuring wet wt, dry wt, and histological examination. After 72 hr of exposure to 3 U/ml krill enzymes, the dry wt of the specimens was reduced to 2.7% ± 1.9 (SEM, n = 5), compared with 31.0% ± 2.7 for placebo, 25.7% ± 2.5 for 120 U/ml papain, and 24.5% ± 3.3 for 2.5/1500 E/ml fibrinolysin/DNAse. The differences between krill enzymes and fibrinolysin/DNAse, papain, and control solution were statistically significant (p < 0.007). These data suggest that krill enzymes are more active than other commonly available proteolytic agents used for wound debridement.
KW - Elase
KW - Fibrinolysin/DNAse
KW - Krill enzymes
KW - Papain
KW - Wound debridement
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U2 - 10.1016/S1357-2725(96)00168-9
DO - 10.1016/S1357-2725(96)00168-9
M3 - Article
C2 - 9363648
AN - SCOPUS:0031009003
SN - 1357-2725
VL - 29
SP - 703
EP - 706
JO - International Journal of Biochemistry and Cell Biology
JF - International Journal of Biochemistry and Cell Biology
IS - 4
ER -