In vitro translation of a 180,000-dalton rous sarcoma virus precursor polypeptide containing both the DNA polymerase and the group-specific antigens

J. McGinnis, A. Hizi, R. E. Smith, J. P. Leis*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Translation of 34S RNA from the Prague C strain of Rous sarcoma virus RSV (Pr-C) in a Kreb's ascites cell-free protein synthesizing system leads to synthesis of three detectable viral precursor polypeptides with electrophoretic mobilities corresponding to molecular weights of about 180,000, 70,000, and 65,000 daltons. The 180,000-dalton protein is precipitated by antiserum directed against purified 13,77 avian sarcoma virus reverse transcriptase as well as by serum against AMV group-specific (gs) antigens, but is not precipitated by serum directed against RSV (Pr-C) gp85. The 70,000- and 65,000-dalton proteins are precipitated by antiserum directed against gs antigens but not by antisera directed against polymerase, MuLV (Friend) p30, or RSV (Pr-C) gp85. Competition experiments with purified polymerase and gs antigens indicate that the 180,000-dalton protein has separate determinants for the gs antigens and polymerase.

Original languageEnglish (US)
Pages (from-to)518-522
Number of pages5
JournalVirology
Volume84
Issue number2
DOIs
StatePublished - Feb 1978

ASJC Scopus subject areas

  • Virology

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