In vitro translation of B-cell stimulatory factor-1 in Xenopus laevis oocytes

Melissa A. Brown*, Cynthia Watson, Junichi Ohara, William E. Paul

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

B-cell stimulatory factor-1 (BSF-1) can be translated in vitro in Xenopus laevis oocytes. This activity is blocked by an antibody to BSF-1. The RNA species coding for BSF-1 activity sediments of ~8-9S and is separable from RNA coding for interleukin-2 activity which sediments at ~ 11.5S. Finally, the fact that BSF-1 can be translated in vitro confirms that functions attributed to BSF-1 do not depend on contamination with other biologically active molecules such as phorbol myristate acetate.

Original languageEnglish (US)
Pages (from-to)538-543
Number of pages6
JournalCellular Immunology
Volume98
Issue number2
DOIs
StatePublished - Apr 1 1986

ASJC Scopus subject areas

  • Immunology

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