In vivo and in vitro characterization of a Plasmodium liver stage-specific promoter

Mariana De Niz, Susanne Helm, Sebastian Horstmann, Takeshi Annoura, Hernando A. Del Portillo, Shahid M. Khan, Volker T. Heussler

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Little is known about stage-specific gene regulation in Plasmodium parasites, in particular the liver stage of development. We have previously described in the Plasmodium berghei rodent model, a liver stage-specific (lisp2) gene promoter region, in vitro. Using a dual luminescence system, we now confirm the stage specificity of this promoter region also in vivo. Furthermore, by substitution and deletion analyses we have extended our in vitro characterization of important elements within the promoter region. Importantly, the dual luminescence system allows analyzing promoter constructs avoiding mouse-consuming cloning procedures of transgenic parasites. This makes extensive mutation and deletion studies a reasonable approach also in the malaria mouse model. Stage-specific expression constructs and parasite lines are extremely valuable tools for research on Plasmodium liver stage biology. Such reporter lines offer a promising opportunity for assessment of liver stage drugs, characterization of genetically attenuated parasites and liver stage-specific vaccines both in vivo and in vitro, and may be key for the generation of inducible systems.

Original languageEnglish (US)
Article numbere0123473
JournalPloS one
Volume10
Issue number4
DOIs
StatePublished - Apr 15 2015

ASJC Scopus subject areas

  • General Agricultural and Biological Sciences
  • General
  • General Biochemistry, Genetics and Molecular Biology

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