In vivo and in vitro selective targeting of the Retinal Pigment Epithelium using a Laser-Scanning Device

Clemens Alt, Carsten Framme, Susanne Schnell, Georg Schuele, Ralf Brinkmann, Charles P. Lin

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Background: Laser photocoagulation is a well-established treatment modality for a variety of retinal disorders, but is difficult to use near the fovea due to thermal retinal destruction. Certain diseases, such as drusen maculopathy, are thought to be caused by a dysfunction of the Retinal Pigment Epithelium. For those diseases selective targeting of the RPE, sparing the adjoining photoreceptors, might be the appropriate treatment to avoid laser scotoma, as it has been shown with application of a train of μs laser pulses by Birngruber and Roider. Material and methods: Our new approach is to use a conventional green cw laser and rapidly scan a small laser spot over the retina so as to produce μs-illumination at each RPE cell. Two scanning devices were developed using acousto-optic deflectors. Results: For the in vitro experiments the ED50 value RPE cell damage was 170 mW with 100 exposures, scanning with a speed of 1 spot diameter/3 μs. In vivo experiments demonstrated an angiographic ED50 threshold of 66 mW for 100 exposures while scanning with an effective illumination time of 5 μs. The ophthalmoscopic threshold was higher than a factor of 2 times the angiographic ED50. Using separated scan lines we show selectivity in the form of surviving cells in between irradiated lines. Conclusion: Selective destruction of RPE cells is possible using laser-scanning devices.

Original languageEnglish (US)
Pages (from-to)59-63
Number of pages5
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume4611
DOIs
StatePublished - Jan 1 2002

Keywords

  • Melanosomes
  • Retinal Pigment Epithelium
  • Scanning laser
  • Selective photocoagulation

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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