TY - JOUR
T1 - In vivo behavior of genetically engineered herpes simplex viruses r7017 and r7020
T2 - Construction and evaluation in rodents
AU - Meignier, Bernard
AU - Longnecker, Richard
AU - Roizman, Bernard
N1 - Funding Information:
Received for publication 1 February 1988and in revised form 19 April 1988. Dr. Longnecker is a postdoctoral trainee supported by grant CA-09241-09from the training program in Viral Oncology, National Cancer Institute. Wethank Marie-Clotilde Bernard, Odette Delisle,ThereseMarie Jourdier, and Denise Vorel (Institut Merieux) for assistance. Please address requests for reprints to Dr. Bernard Roizman, the Marjorie B.KovlerViralOncology Laboratories, the University of Chicago, 910 East 58th Street, Chicago, Illinois 60637.
PY - 1988/9
Y1 - 1988/9
N2 - The herpes simplex virus (HSV) recombinant R7017 was constructed from HSV-1 (strain F) by deleting a portion of the thymidine kinase (tk) gene and by replacing the sequences representing the internal inverted repeats and adjacent genes in the L component with a fragment of the HSV-2 genome encoding the glycoproteins G, D, I, and a portion of E. In addition, the R7020 recombinant contains an HSV-1 DNA fragment encoding the tk gene fused to the a4 gene promoter. The results of studies in mice, guinea pigs, and rabbits were as follows: Both recombinants remained unchanged after nine serial, intracerebral passages in mice; the recombinants could not be differentiated with respect to attenuation in mice injected intracerebrally, in vaginally infected guinea pigs, and in rabbits inoculated on the scarified cornea. Given intradermally or intramuscularly, the recombinants prevented severe infections by virulent challenge viruses, and R7020 established latent infections (at a low frequency) in all species tested, whereas latent R7017 virus was detected in rabbits only.
AB - The herpes simplex virus (HSV) recombinant R7017 was constructed from HSV-1 (strain F) by deleting a portion of the thymidine kinase (tk) gene and by replacing the sequences representing the internal inverted repeats and adjacent genes in the L component with a fragment of the HSV-2 genome encoding the glycoproteins G, D, I, and a portion of E. In addition, the R7020 recombinant contains an HSV-1 DNA fragment encoding the tk gene fused to the a4 gene promoter. The results of studies in mice, guinea pigs, and rabbits were as follows: Both recombinants remained unchanged after nine serial, intracerebral passages in mice; the recombinants could not be differentiated with respect to attenuation in mice injected intracerebrally, in vaginally infected guinea pigs, and in rabbits inoculated on the scarified cornea. Given intradermally or intramuscularly, the recombinants prevented severe infections by virulent challenge viruses, and R7020 established latent infections (at a low frequency) in all species tested, whereas latent R7017 virus was detected in rabbits only.
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U2 - 10.1093/infdis/158.3.602
DO - 10.1093/infdis/158.3.602
M3 - Article
C2 - 2842408
AN - SCOPUS:0023777661
VL - 158
SP - 602
EP - 614
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
SN - 0022-1899
IS - 3
ER -