In vivo binding of active heat shock transcription factor 1 to human chromosome 9 heterochromatin during stress

Caroline Jolly*, Lara Konecny, Deborah L. Grady, Yulia A. Kutskova, José J. Cotto, Richard I. Morimoto, Claire Vourc'h

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

89 Scopus citations

Abstract

Activation of the mammalian heat shock transcription factor (HSF)1 by stress is a multistep process resulting in the transcription of heat shock genes. Coincident with these events is the rapid and reversible redistribution of HSF1 to discrete nuclear structures termed HSF1 granules, whose function is still unknown. Key features are that the number of granules correlates with cell ploidy, suggesting the existence of a chromosomal target. Here we show that in humans, HSF1 granules localize to the 9q11-q12 heterochromatic region. Within this locus, HSF1 binds through direct DNA-protein interaction with a nucleosome-containing subclass of satellite III repeats. HSF1 granule formation only requires the DNA binding competence and the trimerization of the factor. This is the first example of a transcriptional activator that accumulates transiently and reversibly on a chromosome-specific heterochromatic locus.

Original languageEnglish (US)
Pages (from-to)775-781
Number of pages7
JournalJournal of Cell Biology
Volume156
Issue number5
DOIs
StatePublished - Mar 4 2002

Keywords

  • HSF1 granules
  • Heterochromatin
  • Nucleus
  • Satellite III
  • Stress

ASJC Scopus subject areas

  • Cell Biology

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