In vivo corneal neovascularization imaging by optical-resolution photoacoustic microscopy

Wenzhong Liu; Kathryn M. Schultz; Kevin Zhang; Amy Sasman; Fengli Gao; Tsutomu Kume; Hao F. Zhang

doi:10.1016/j.pacs.2014.04.003

In vivo corneal neovascularization imaging by optical-resolution photoacoustic microscopy

Wenzhong Liu, Kathryn M. Schultz, Kevin Zhang, Amy Sasman, Fengli Gao, Tsutomu Kume^*, Hao F. Zhang

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Corneal neovascularization leads to blurred vision, thus in vivo visualization is essential for pathological studies in animal models. Photoacoustic (PA) imaging can delineate microvasculature and hemodynamics noninvasively, which is suitable for investigating corneal neovascularization. In this study, we demonstrate in vivo imaging of corneal neovascularization in the mouse eye by optical-resolution photoacoustic microscopy (OR-PAM), where corneal neovascularization is induced by deliberate alkali burn injuries in C57BL6/J inbred mice corneas on the left eye. We used OR-PAM to image five mice with corneal alkali burn injuries; the uninjured eyes (right eye) in these mice are then used as the controls. Corneal images acquired by OR-PAM with and without alkali burn injury are compared, clear signs of corneal neovascularization are present in the OR-PAM images of injured eyes; the OR-PAM results are also confirmed by postmortem fluorescence-labeled confocal microscopy.

Original language	English (US)
Pages (from-to)	81-86
Number of pages	6
Journal	Photoacoustics
Volume	2
Issue number	2
DOIs	https://doi.org/10.1016/j.pacs.2014.04.003
State	Published - Jun 2014

Funding

The authors acknowledge the generous financial supports from NIH grants HL074121 , HL108795 and EY019484 to TK, NIH grants 1R01EY019951 and 1RC4EY021357 to HFZ, and NSF grant CBET-1055379 to HFZ. Wenzhong Liu is a Howard Hughes Medical Institute (HHMI) international student fellow.

Keywords

Cornea
Neovascularization
Photoacoustic microscopy

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

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10.1016/j.pacs.2014.04.003

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title = "In vivo corneal neovascularization imaging by optical-resolution photoacoustic microscopy",

abstract = "Corneal neovascularization leads to blurred vision, thus in vivo visualization is essential for pathological studies in animal models. Photoacoustic (PA) imaging can delineate microvasculature and hemodynamics noninvasively, which is suitable for investigating corneal neovascularization. In this study, we demonstrate in vivo imaging of corneal neovascularization in the mouse eye by optical-resolution photoacoustic microscopy (OR-PAM), where corneal neovascularization is induced by deliberate alkali burn injuries in C57BL6/J inbred mice corneas on the left eye. We used OR-PAM to image five mice with corneal alkali burn injuries; the uninjured eyes (right eye) in these mice are then used as the controls. Corneal images acquired by OR-PAM with and without alkali burn injury are compared, clear signs of corneal neovascularization are present in the OR-PAM images of injured eyes; the OR-PAM results are also confirmed by postmortem fluorescence-labeled confocal microscopy.",

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author = "Wenzhong Liu and Schultz, {Kathryn M.} and Kevin Zhang and Amy Sasman and Fengli Gao and Tsutomu Kume and Zhang, {Hao F.}",

note = "Funding Information: The authors acknowledge the generous financial supports from NIH grants HL074121 , HL108795 and EY019484 to TK, NIH grants 1R01EY019951 and 1RC4EY021357 to HFZ, and NSF grant CBET-1055379 to HFZ. Wenzhong Liu is a Howard Hughes Medical Institute (HHMI) international student fellow. ",

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AU - Sasman, Amy

AU - Gao, Fengli

AU - Kume, Tsutomu

AU - Zhang, Hao F.

N1 - Funding Information: The authors acknowledge the generous financial supports from NIH grants HL074121 , HL108795 and EY019484 to TK, NIH grants 1R01EY019951 and 1RC4EY021357 to HFZ, and NSF grant CBET-1055379 to HFZ. Wenzhong Liu is a Howard Hughes Medical Institute (HHMI) international student fellow.

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N2 - Corneal neovascularization leads to blurred vision, thus in vivo visualization is essential for pathological studies in animal models. Photoacoustic (PA) imaging can delineate microvasculature and hemodynamics noninvasively, which is suitable for investigating corneal neovascularization. In this study, we demonstrate in vivo imaging of corneal neovascularization in the mouse eye by optical-resolution photoacoustic microscopy (OR-PAM), where corneal neovascularization is induced by deliberate alkali burn injuries in C57BL6/J inbred mice corneas on the left eye. We used OR-PAM to image five mice with corneal alkali burn injuries; the uninjured eyes (right eye) in these mice are then used as the controls. Corneal images acquired by OR-PAM with and without alkali burn injury are compared, clear signs of corneal neovascularization are present in the OR-PAM images of injured eyes; the OR-PAM results are also confirmed by postmortem fluorescence-labeled confocal microscopy.

AB - Corneal neovascularization leads to blurred vision, thus in vivo visualization is essential for pathological studies in animal models. Photoacoustic (PA) imaging can delineate microvasculature and hemodynamics noninvasively, which is suitable for investigating corneal neovascularization. In this study, we demonstrate in vivo imaging of corneal neovascularization in the mouse eye by optical-resolution photoacoustic microscopy (OR-PAM), where corneal neovascularization is induced by deliberate alkali burn injuries in C57BL6/J inbred mice corneas on the left eye. We used OR-PAM to image five mice with corneal alkali burn injuries; the uninjured eyes (right eye) in these mice are then used as the controls. Corneal images acquired by OR-PAM with and without alkali burn injury are compared, clear signs of corneal neovascularization are present in the OR-PAM images of injured eyes; the OR-PAM results are also confirmed by postmortem fluorescence-labeled confocal microscopy.

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KW - Neovascularization

KW - Photoacoustic microscopy

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In vivo corneal neovascularization imaging by optical-resolution photoacoustic microscopy

Abstract

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Keywords

ASJC Scopus subject areas

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