In vivo corneal neovascularization imaging by optical-resolution photoacoustic microscopy

Wenzhong Liu, Kathryn M. Schultz, Kevin Zhang, Amy Sasman, Fengli Gao, Tsutomu Kume*, Hao F. Zhang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


Corneal neovascularization leads to blurred vision, thus in vivo visualization is essential for pathological studies in animal models. Photoacoustic (PA) imaging can delineate microvasculature and hemodynamics noninvasively, which is suitable for investigating corneal neovascularization. In this study, we demonstrate in vivo imaging of corneal neovascularization in the mouse eye by optical-resolution photoacoustic microscopy (OR-PAM), where corneal neovascularization is induced by deliberate alkali burn injuries in C57BL6/J inbred mice corneas on the left eye. We used OR-PAM to image five mice with corneal alkali burn injuries; the uninjured eyes (right eye) in these mice are then used as the controls. Corneal images acquired by OR-PAM with and without alkali burn injury are compared, clear signs of corneal neovascularization are present in the OR-PAM images of injured eyes; the OR-PAM results are also confirmed by postmortem fluorescence-labeled confocal microscopy.

Original languageEnglish (US)
Pages (from-to)81-86
Number of pages6
Issue number2
StatePublished - Jun 2014


  • Cornea
  • Neovascularization
  • Photoacoustic microscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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