In vivo establishment of T98G human glioblastoma

Marvin Rubenstein*, M. Shaw, Y. Mirochnik, L. Slobodskoy, R. Glick, T. Lichtor, P. Chou, P. Guinan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Human derived T98G glioblastoma has long been utilized as an in vitro model for epidermal growth factor receptor (EGFR)-mediated growth regulation. Recently, T98G has been employed to develop new types of therapy directed at limiting EGFR expression such as by administration of antisense oligonucleotides directed against EGFR encoding mRNA. A major limitation to extending this model for in vivo application is that T98G implanted s.c. or intracerebrally has been reported not to grow in nude mice. In an effort to extend this model to permit in vivo studies, we evaluated the use of Matrigel and orthotopic (intracranial) implantation techniques. When equal volumes of Matrigel were mixed with T98G cell suspensions, tumors developed at both flank and orthotopic locations. Four groups of nude mice were inoculated into the flanks with either 105, 106, 4 x 106 or 107 T98G cells in a 150 μl total volume with Matrigel. In 1/5, 3/5, 1/5 and 1/3 mice receiving 105, 106, 4 x 106 and 107 cells, respectively, tumors developed 11, 15, 15 and 15 weeks, respectively, following inoculation. Out of 4 mice inoculated orthotopically (intracranially into the frontal lobe) with only 4 x 104 cells and Matrigel, 2 developed tumors. However, all mice (4/4) inoculated orthotopically with 4 x 105 cells in a 10 μl total volume with Matrigel developed tumors. Two were identified histologically following a scheduled sacrifice at 36 and 60 days and two more at 103 and 118 days after sacrifice following abnormal behavior. The best tumor establishment efficacy combined orthotopic implantation of 4 x 105 T98G cells with Matrigel. These techniques permit the use of T98G glioblastoma as an in vivo model for new forms of therapy.

Original languageEnglish (US)
Pages (from-to)391-393
Number of pages3
JournalMethods and Findings in Experimental and Clinical Pharmacology
Volume21
Issue number6
DOIs
StatePublished - Jul 27 1999

Keywords

  • Brain tumors
  • T98G glioblastoma

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

Fingerprint Dive into the research topics of 'In vivo establishment of T98G human glioblastoma'. Together they form a unique fingerprint.

Cite this