In vivo expression and variation of Escherichia coli type 1 and P pili in the urine of adults with acute urinary tract infections

P. V. Kisielius, W. R. Schwan, S. K. Amundsen, J. L. Duncan, A. J. Schaeffer

Research output: Contribution to journalArticlepeer-review

58 Scopus citations

Abstract

In vivo expression of pili by Escherichia coli in the urine of 41 adults with lower urinary tract infections was analyzed by immunostaining with polyclonal antiserum to type 1 and P pili. Type 1 pili were detected in 31 of 41 urine specimens, while P pili were detected in 6 of 18 specimens. The piliation status of bacterial populations in urine was heterogeneous, varying from predominantly piliated to a mixture of piliated and nonpiliated cells. Bacteria frequently adhered to exfoliated uroepithelial cells and leukocytes in urine. Expression of pili in vivo did not always correlate with the hemagglutination phenotype after growth in vitro. Strains isolated from different sites in the urogenital tract of two individuals showed phenotypic variation in the state of piliation. The results demosntrate that E. coli type 1 and P pili are expressed and are subject to variation in vivo during acute urinary tract infections in adults.

Original languageEnglish (US)
Pages (from-to)1656-1662
Number of pages7
JournalInfection and immunity
Volume57
Issue number6
StatePublished - 1989

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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