In vivo gene therapy with interleukin-12 inhibits primary vascular tumor growth and induces apoptosis in a mouse model

Chong Wang, M. Eugenia Quevedo, Brian J. Lannutti, Kenneth B. Gordon, Guo Danqing, Sun Wenn, Amy S. Paller*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

Interleukin-12 is proposed to have anti-neoplastic activity on the basis of both its anti-angiogenic and immunologic effects. Gene gun therapy with interleukin-12 cDNA into the peritumoral area of immunocompetent 129/J mice with life-threatening primary vascular tumors reduced tumor volume 7.5-fold and almost tripled the duration of mouse survival, in contrast with luciferase-bombarded control mice. Epidermal expression of mouse interleukin- 12 elevated tumoral and serum levels of interferon-γ and tumor necrosis factor-α, increased the tumoral populations of T lymphocyte and natural killer cells, and induced tumor apoptosis. Gene transfer of interleukin-12 had little effect on tumor volumes and survival of tumor-bearing athymic nude mice, emphasizing the requirement for T cell directed cellular immunity. Peritumoral gene gun introduction of interleukin-12 may be a novel, cost- effective approach to limit the growth and associated mortality of life- threatening tumors.

Original languageEnglish (US)
Pages (from-to)775-781
Number of pages7
JournalJournal of Investigative Dermatology
Volume112
Issue number5
DOIs
StatePublished - 1999

Keywords

  • Anti-neoplastic agents
  • Gene therapy
  • Hemangioendothelioma
  • Hemangioma
  • Interleukin-12

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Dermatology
  • Cell Biology

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