In vivo imaging of the inner retinal layer structure in mice after eye-opening using visible-light optical coherence tomography

Lisa Beckmann, Zhen Cai, James Cole, David A. Miller, Mingna Liu, Marta Grannonico, Xian Zhang, Hyun Jung Ryu, Peter A. Netland, Xiaorong Liu, Hao F. Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

The growth of the mouse eye and retina after birth is a dynamic, highly regulated process. In this study, we applied visible-light optical coherence tomography (vis-OCT), a non-invasive imaging technique, to examine developing retinal layer structures after eye-opening. We introduced a resampled circumpapillary B-scan averaging technique to improve the inter-layer contrast, enabling retinal layer thickness measurements as early as postnatal day 13 (P13) – right after eye-opening. We confirmed vis-OCT measurements using ex vivo confocal microscopy of retinal sections at different ages. Our results demonstrate that vis-OCT can visualize the developmental murine retinal layer structure in vivo, which offers us new opportunities to better characterize the pathological alterations in mouse models of developmental eye diseases.

Original languageEnglish (US)
Article number108756
JournalExperimental eye research
Volume211
DOIs
StatePublished - Oct 2021

Keywords

  • In vivo imaging
  • Optical coherence tomography
  • Retinal development

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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