In vivo measurement of human wrist extensor muscle sarcomere length changes

R. L. Lieber*, G. J. Loren, J. Friden

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

147 Scopus citations

Abstract

1. Human extensor carpi radialis brevis (ECRB) sarcomere length was measured intraoperatively in five subjects using laser diffraction. 2. In a separate cadaveric study, ECRB tendons were loaded to the muscle's predicted maximum tetanic tension, and tendon strain was measured to estimate active sarcomere shortening at the expense of tendon lengthening. 3. As the wrist joint was passively flexed from full extension to full flexion, ECRB sarcomere length increased from 2.6 to 3.4 μm at a rate of 7.6 nm/deg joint angle rotation. Correcting for tendon elongation during muscle activation yielded an active sarcomere length range of 2.44 to 3.33 μm. Maximal predicted sarcomere shortening accompanying muscle activation was dependent on initial sarcomere length and was always <0.15 μm, suggesting a minimal effect of tendon compliance. 4. Thin filament lengths measured from electron micrographs of muscle biopsies obtained from the same region of the ECRB muscles were 1.30 ± 0.27 (SE) μm whereas thick filaments were 1.66 ± .027 μm long, suggesting an optimal sarcomere length of 2.80 μm and a maximum sarcomere length for active force generation of 4.26 μm. 5. These experiments demonstrate that human skeletal muscles can function on the descending limb of their sarcomere length-tension relationship under physiological conditions. Thus, muscle force changes during joint rotation are an important component of the motor control system.

Original languageEnglish (US)
Pages (from-to)874-881
Number of pages8
JournalJournal of neurophysiology
Volume71
Issue number3
DOIs
StatePublished - 1994

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology

Fingerprint Dive into the research topics of 'In vivo measurement of human wrist extensor muscle sarcomere length changes'. Together they form a unique fingerprint.

Cite this