@article{bf68f65d7c44477c87583bee2cdce0a7,
title = "In vivo migration of endogenous brain progenitor cells guided by an injectable peptide amphiphile biomaterial",
abstract = "Biomaterials hold great promise in helping the adult brain regenerate and rebuild after trauma. Peptide amphiphiles (PAs) are highly versatile biomaterials, gelling and forming macromolecular structures when exposed to physiological levels of electrolytes. We are here reporting on the first ever in vivo use of self-assembling PA carrying a Tenascin-C signal (E2Ten-C PA) for the redirection of endogenous neuroblasts in the rodent brain. The PA forms highly aligned nanofibers, displaying the migratory sequence of Tenascin-C glycoprotein as epitope. In this in vivo work, we have formed in situ a gel of aligned PA nanofibers presenting a migratory Tenascin-C signal sequence in the ventral horn of the rostral migratory stream, creating a track reaching the neocortex. Seven days posttransplant, doublecortin positive cells were observed migrating inside and alongside the injected biomaterial, reaching the cortex. We observed a 24-fold increase in number of redirected neuroblasts for the E2Ten-C PA–injected animals compared to control. We also found injecting the E2Ten-C PA to cause minimal neuroinflammatory response. Analysing GFAP+ astrocytes and Iba1+ microglia activation, the PA does not elicit a stronger neuroinflammatory response than would be expected from a small needle stab wound. Redirecting endogenous neuroblasts and increasing the number of cells reaching a site of injury using PAs may open up new avenues for utilizing the pool of neuroblasts and neural stem cells within the adult brain for regenerating damaged brain tissue and replacing neurons lost to injury.",
keywords = "Tenascin-C, cell migration, doublecortin, nanofiber hydrogel, peptide amphiphile, rostral migratory stream",
author = "Reza Motalleb and Berns, {Eric J.} and Piyush Patel and Julie Gold and Stupp, {Samuel I.} and Kuhn, {H. Georg}",
note = "Funding Information: Swedish Medical Research Council (Vetenskapsr{\aa}det), Grant/Award Number: K2015‐63X‐20117‐10‐4; the Swedish Federal Government under the LUA/ALF agreement, Grant/Award Number: ALFGBG‐45096; Swedish Brain Foundation (Hj{\"a}rnfonden); Stroke‐Riksf{\"o}rbundet; National Institutes of Health/National Institute of Biomedical Imaging and Bioengineering, Grant/Award Number: 5R01EB003806‐07; Center for Regenerative Nanomedicine at the Simpson Querrey Institute for BioNanotechnology at Northwestern Funding Information: This project was supported by Swedish Medical Research Council (Vetenskapsr{\aa}det) grant K2015-63X-20117-10-4, the Swedish Federal Government under the LUA/ALF agreement ALFGBG-450961, Swedish Brain Foundation (Hj{\"a}rnfonden), Stroke-Riksf{\"o}rbundet, National Institutes of Health/National Institute of Biomedical Imaging and Bioengineering Award Number 5R01EB003806-07, and by the Center for Regenerative Nanomedicine at the Simpson Querrey Institute for BioNanotechnology at Northwestern. Peptide synthesis was performed in the Peptide Synthesis Core Facility of the Simpson Querrey Institute at Northwestern University. The U.S. Army Research Office, the U.S. Army Medical Research and Materiel Command, the Department of Energy, the Frederick S. Upton Foundation, and Northwestern University provided funding to develop this facility. Funding Information: This project was supported by Swedish Medical Research Council (Vetenskapsr{\aa}det) grant K2015‐63X‐20117‐10‐4, the Swedish Federal Government under the LUA/ALF agreement ALFGBG‐450961, Swedish Brain Foundation (Hj{\"a}rnfonden), Stroke‐Riksf{\"o}rbundet, National Institutes of Health/National Institute of Biomedical Imaging and Bioengineering Award Number 5R01EB003806‐07, and by the Center for Regenerative Nanomedicine at the Simpson Querrey Institute for BioNanotechnology at Northwestern. Peptide synthesis was performed in the Peptide Synthesis Core Facility of the Simpson Querrey Institute at Northwestern University. The U.S. Army Research Office, the U.S. Army Medical Research and Materiel Command, the Department of Energy, the Frederick S. Upton Foundation, and Northwestern University provided funding to develop this facility. Publisher Copyright: Copyright {\textcopyright} 2018 John Wiley & Sons, Ltd.",
year = "2018",
month = apr,
doi = "10.1002/term.2644",
language = "English (US)",
volume = "12",
pages = "e2123--e2133",
journal = "Journal of Tissue Engineering and Regenerative Medicine",
issn = "1932-6254",
publisher = "John Wiley and Sons Ltd",
number = "4",
}