In vivo multiphoton microscopy of deep tissue with gradient index lenses

Michael J. Levene*, Daniel A. Dombeck, Rebecca M. Williams, Jesse Skoch, Gregory A. Hickey, Karl A. Kasischke, Raymond P. Molloy, Martin Ingelsson, Edward A. Stern, Jochen Klucken, Brian J. Bacskai, Warren R. Zipfel, Bradley T. Hyman, W. W. Webb

*Corresponding author for this work

Research output: Contribution to journalConference articlepeer-review

1 Scopus citations


Gradient index lenses enable multiphoton microscopy of deep tissues in the intact animal. In order to assess their applicability to clinical research, we present in vivo multiphoton microscopy with gradient index lenses in brain regions associated with Alzheimer's disease and Parkinson's disease in both transgenic and wild-type mice. We also demonstrate microscopy of ovary in wild type mouse using only intrinsic fluorescence and second harmonic generation, signal sources which may prove useful for both the study and diagnosis of cancer.

Original languageEnglish (US)
Pages (from-to)291-296
Number of pages6
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - Oct 27 2004
EventProgress in Biomedical Optics and Imaging - Multiphoton Microscopy in the Biomedical Sciences IV - San Jose, CA, United States
Duration: Jan 25 2004Jan 27 2004

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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