In vivo outcomes of tissue-engineered osteochondral grafts

B. Sonny Bal*, Mohamed N. Rahaman, Prakash Jayabalan, Keiichi Kuroki, Mary K. Cockrell, Jian Q. Yao, James L. Cook

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

Tissue-engineered osteochondral grafts have been synthesized from a variety of materials, with some success at repairing chondral defects in animal models. We hypothesized that in tissue-engineered osteochondral grafts synthesized by bonding mesenchymal stem cellloaded hydrogels to a porous material, the choice of the porous scaffold would affect graft healing to host bone, and the quality of cell restoration at the hyaline cartilage surface. Bone marrow-derived allogeneic mesenchymal stem cells were suspended in hydrogels that were attached to cylinders of porous tantalum metal, allograft bone, or a bioactive glass. The tissueengineered osteochondral grafts, thus created were implanted into experimental defects in rabbit knees. Subchondral bone restoration, defect fill, bone ingrowth-implant integration, and articular tissue quality were compared between the three subchondral materials at 6 and 12 weeks. Bioactive glass and porous tantalum were superior to bone allograft in integrating to adjacent host bone, regenerating hyaline-like tissue at the graft surface, and expressing type II collagen in the articular cartilage.

Original languageEnglish (US)
Pages (from-to)164-174
Number of pages11
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume93
Issue number1
DOIs
StatePublished - Apr 1 2010

Keywords

  • Animal model
  • Bone restoration
  • Mesenchymal stem cells
  • Osteochondral grafts
  • Tissue engineering

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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