Abstract
Tissue-engineered osteochondral grafts have been synthesized from a variety of materials, with some success at repairing chondral defects in animal models. We hypothesized that in tissue-engineered osteochondral grafts synthesized by bonding mesenchymal stem cellloaded hydrogels to a porous material, the choice of the porous scaffold would affect graft healing to host bone, and the quality of cell restoration at the hyaline cartilage surface. Bone marrow-derived allogeneic mesenchymal stem cells were suspended in hydrogels that were attached to cylinders of porous tantalum metal, allograft bone, or a bioactive glass. The tissueengineered osteochondral grafts, thus created were implanted into experimental defects in rabbit knees. Subchondral bone restoration, defect fill, bone ingrowth-implant integration, and articular tissue quality were compared between the three subchondral materials at 6 and 12 weeks. Bioactive glass and porous tantalum were superior to bone allograft in integrating to adjacent host bone, regenerating hyaline-like tissue at the graft surface, and expressing type II collagen in the articular cartilage.
Original language | English (US) |
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Pages (from-to) | 164-174 |
Number of pages | 11 |
Journal | Journal of Biomedical Materials Research - Part B Applied Biomaterials |
Volume | 93 |
Issue number | 1 |
DOIs | |
State | Published - Apr 2010 |
Keywords
- Animal model
- Bone restoration
- Mesenchymal stem cells
- Osteochondral grafts
- Tissue engineering
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials