In vivo simultaneous evaluations of sarcomere imaging and muscle fiber tension

Yi Ning Wu, Yupeng Ren, Liang Ching Tsai, Fan Gao, Li Qun Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Muscle fiber tension and sarcomere length play critical roles in regulating muscle functions and adaptations under pathological conditions. However, methods are lacking to quantify these two variables simultaneously in vivo. A novel force microscope was developed with the unique capabilities of estimating muscle fiber tension and acquiring sarcomere images simultaneously in vivo. The force microscope consisting of a custom microscopic imaging system and a force sensor was used to quantify in vivo sarcomere length, muscle fiber tension and stress of the tibialis cranialis muscle at plantar-flexed and dorsi-flexed positions from 11 rat hind limbs. Results showed that sarcomere images and fiber tension could be measured together in vivo with significantly higher muscle fiber tension and stress and longer sarcomere length at the plantar-flexed position when compared to their counterparts at the dorsi-flexed position. The fiber tension estimated using the force microscope had close agreement with the direct measurements of the fiber tension. The present force microscope with simultaneous characterizations of fiber tension and sarcomere imaging provides us a useful in vivo tool to investigate the roles of muscle tension in regulating sarcomere and muscle fiber functions under physiological and pathological conditions.

Original languageEnglish (US)
Pages (from-to)797-801
Number of pages5
JournalJournal of Biomechanics
Issue number5
StatePublished - Mar 21 2016


  • Force microscope
  • Muscle fiber
  • Sarcomere

ASJC Scopus subject areas

  • Biophysics
  • Rehabilitation
  • Biomedical Engineering
  • Orthopedics and Sports Medicine


Dive into the research topics of 'In vivo simultaneous evaluations of sarcomere imaging and muscle fiber tension'. Together they form a unique fingerprint.

Cite this