In vivo two-photon imaging measuring the blood-brain barrier permeability during early postnatal brain development in rodent

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

The blood-brain barrier (BBB) is a unique structure between the cerebral blood circulation and the delicate neural environment that is important in regulating the movement of molecules and ions involved in brain development and function. However, little is known about the physiological permeability of molecules and ions across the BBB during brain development. In this study we applied an innovative approach to examine the development of BBB properties quantitatively. Two-photon microscopy was employed to measure BBB permeability in real time in vivo. Vascular growth and specific interactions between astrocyte end feet and microvessels were studied by using a combination of IB4 histochemistry, immunohistochemistry, confocal microscopy and 3D analysis.

Original languageEnglish (US)
Title of host publicationMultiphoton Microscopy in the Biomedical Sciences XVI
EditorsPeter T. C. So, Karsten Konig, Ammasi Periasamy, Karsten Konig
PublisherSPIE
ISBN (Electronic)9781628419467
DOIs
StatePublished - 2016
EventMultiphoton Microscopy in the Biomedical Sciences XVI - San Francisco, United States
Duration: Feb 14 2016Feb 16 2016

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9712
ISSN (Print)1605-7422

Conference

ConferenceMultiphoton Microscopy in the Biomedical Sciences XVI
Country/TerritoryUnited States
CitySan Francisco
Period2/14/162/16/16

Keywords

  • Two-Photon imaging
  • astrocytes
  • blood-brain barrier
  • cerebral microvessel
  • immunohistochemistry
  • permeability
  • postnatal brain development

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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