In vivo volumetric imaging of subcutaneous microvasculature by photoacoustic microscopy

Hao F. Zhang*, Konstantin Maslov, Meng N. Li, George Stoica, Lihong V. Wang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

110 Scopus citations

Abstract

Photoacoustic microscopy was developed to achieve volumetric imaging of the anatomy and functions of the subcutaneous microvasculature in both small animals and humans in vivo with high spatial resolution and high signal-to-background ratio. By following the skin contour in raster scanning, the ultrasonic transducer maintains focusing in the region of interest. Furthermore, off-focus lateral resolution is improved by using a synthetic-aperture focusing technique based on the virtual point detector concept. Structural images are acquired in both rats and humans, whereas functional images representing hemoglobin oxygen saturation are acquired in rats. After multiscale vesselness filtering, arterioles and venules in the image are separated based on the imaged oxygen saturation levels. Detailed structural information, such as vessel depth and spatial orientation, are revealed by volume rendering.

Original languageEnglish (US)
Pages (from-to)9317-9323
Number of pages7
JournalOptics Express
Volume14
Issue number20
DOIs
StatePublished - Oct 2006

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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