Inactivation of AKT induces cellular senescence in uterine leiomyoma

Xiaofei Xu, Zhenxiao Lu, Wenan Qiang, Vania Vidimar, Beihua Kong, J. Julie Kim*, Jian Jun Wei

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Uterine leiomyomas (fibroids) are a major public health problem. Current medical treatments with GnRH analogs do not provide long-term benefit. Thus, permanent shrinkage or inhibition of fibroid growth via medical means remains a challenge. The AKT pathway is a major growth and survival pathway for fibroids. We propose that AKT inhibition results in a transient regulation of specific mechanisms that ultimately drive cells into cellular senescence or cell death. In this study, we investigated specific mechanisms of AKT inhibition that resulted in senescence. We observed that administration of MK-2206, an allosteric AKT inhibitor, increased levels of reactive oxygen species, up-regulated the microRNA miR-182 and several senescence-associated genes (including p16, p53, p21, and β-galactosidase), and drove leiomyoma cells into stress-induced premature senescence (SIPS). Moreover, induction of SIPS was mediated by HMGA2, which colocalized to senescence-associated heterochromatin foci. This study provides a conceivable molecular mechanism of SIPS by AKT inhibition in fibroids.

Original languageEnglish (US)
Pages (from-to)1510-1519
Number of pages10
JournalEndocrinology
Volume155
Issue number4
DOIs
StatePublished - Apr 2014

ASJC Scopus subject areas

  • Endocrinology

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