Increased expression of intercellular adhesion molecule 1, CD11/CD18 cell surface adhesion glycoproteins and α4β1 integrin in a rat model of chronic interstitial lung fibrosis

N. Barquin, P. Chou, C. Ramos, M. Montano, A. Pardo, M. Selman

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The expression of the intercellular adhesion molecule 1 (ICAM-1), and the integrins CD49, CD1 Vole, and CD1 la (LFA-1α chain) was analyzed in an experimental model of pulmonary fibrosis. Adult rats were exposed to 75% oxygen during 10 weeks, and to 2.0 mg/kg of paraquat twice weekly. Rats were sacrificed at 2 days, and at 2 and 10 weeks after the first injection of paraquat. Lungs were fixed in 4% paraformaldehyde and used for histology and immunohistochemistry. At 2 days the lungs showed a diffuse inflammation composed of a mixed polymorphonuclear and mononuclear cell infiltrate. Afterwards, the inflammatory process was predominantly mononuclear, and an increasing fibroblast proliferation was observed. Early inflammatory events (48 h) correlated with a moderate increased expression of ICAM-1, LFA, and CD1 lb/c in epithelial cells as well as a pronounced expression of ICAM-1 and CD1 lb/c in macrophages. At 2 and 10 weeks, there was a progressive increased expression of CD1 lb/c and ICAM-1 by macrophages, as well as of LFA in epithelial cells, and of ICAM-1 and CD49 by epithelial and interstitial cells. Lymphocytes showed a slight increased expression of LFA at 2 weeks, and of CD49 at 2 and 10 weeks. These results suggest that macrophages expressing ICAM-1, CD1 lb/c, and CD49 are involved in the earlier and late phases of the disease whereas fibroblast and epithelial cells expressing ICAM-1 and CD49 might play a role in the cell interactions involved in the fibrotic phase.

Original languageEnglish (US)
Pages (from-to)187-192
Number of pages6
JournalPathobiology
Volume64
Issue number4
DOIs
StatePublished - Jan 1 1996

Keywords

  • Adhesion molecules
  • Fibrosis
  • ICAM-1
  • Integrins
  • Lung fibrosis

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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