Increased expression of lymphocyte-derived cytokines in benign hyperplastic prostate tissue, identification of the producing cell types, and effect of differentially expressed cytokines on stromal cell proliferation

Gero Kramer, Georg E. Steiner*, Alessandra Handisurya, Ursula Stix, Andrea Haitel, Birgit Knerer, Alois Gessl, Chung Lee, Michael Marberger

*Corresponding author for this work

Research output: Contribution to journalArticle

135 Scopus citations

Abstract

BACKGROUND. Benign prostatic hyperplasia (BPH) frequently exhibit infiltration of CD4 +/CD45RO + memory-T-lymphocytes. Expression and impact of lymphocyte-derived growth factors on prostatic stromal cell (PSC) growth were investigated. METHODS. Lymphokine synthesis in normal prostate tissues (n = 3), BPH-tissues (n = 13), BPH-derived T-cells (n = 6), BPH-derived epithelial cells (BPH-EC) (n = 5), normal prostate-derived (n = 3) and BPH-derived stromal cell lines (BPH-SC) (n = 6), and prostate cancer (CaP) lines (n = 3) was analyzed by RT-PCR and Southern-blotting. The effect of interleukin (IL)-2, -4, -7, and interferon-γ (IFN-γ) on normal and BPH-SC growth was investigated by 2H-thymidine incorporation assays. RESULTS. All BPH-tissues and, to a lesser degree, normal prostates, expressed significant amounts of IFN-γ mRNA. However, only BPH-tissues contained IL-2 and IL-4 mRNA (ratio: 10:13). BPH-T-cell lines were heterogenous in composition and expressed significant amounts of IFN-γ, IL-2, and IL-4 mRNA. Low level expression of these lymphokines was also observed in BPH-EC, CaP lines, and PSC lines. IL-2, -7 and IFN-7 stimulated the proliferation of BPH-PSC lines but not that of normal PSC, while IL-4 inhibited BPH-PSC growth. CONCLUSIONS. Chronic inflammation may induce an increased growth pattern of fibromuscular tissue in BPH similar to that of wound healing.

Original languageEnglish (US)
Pages (from-to)43-58
Number of pages16
JournalProstate
Volume52
Issue number1
DOIs
StatePublished - Jun 1 2002

Keywords

  • BPH-derived T-cells
  • Benign prostatic hyperplasia
  • Cytokines
  • Normal prostate
  • Prostate epithelial cells
  • Prostatic stromal cells

ASJC Scopus subject areas

  • Oncology
  • Urology

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