Increased expression of sulfated glycoprotein-2 and DNA fragmentation in the pancreas of copper-deficient rats

H. Ide, A. V. Yeldandi, J. K. Reddy, M. S. Rao

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17 Scopus citations

Abstract

Recent morphological studies from our laboratory have shown that copper deficiency-induced pancreatic involution in rats is secondary to apoptosis (M. S. Rao, A. V. Yeldandi, V. Subbarao, and J. K. Reddy, 1993, Am. J. Pathol. 142, 1952-1957). To corroborate the morphological findings, we have examined pancreases from copper-deficient rats for expression of sulfated glycoprotein-2 (SGP-2) mRNA and DNA fragmentation, which are considered as specific markers of apoptosis. Agarose gel electrophoresis of the DNA extracted from pancreas of rats maintained on copper deficient diet (CUDD) for 5 and 7 weeks showed characteristic “ladder” pattern, whereas DNA from control rats and rats maintained on CUDD for 3 weeks showed no fragmentation. These findings correlated well with the histological changes. Northern blot analysis of total RNA revealed a marked increase in the expression of SGP-2 mRNA at 5 weeks followed by a gradual decrease at 6 and 7 weeks. These results further support that the mechanism of copper deficiency-induced pancreatic involution is through apoptosis.

Original languageEnglish (US)
Pages (from-to)174-177
Number of pages4
JournalToxicology and Applied Pharmacology
Volume126
Issue number1
DOIs
StatePublished - May 1994

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

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