Increased LIS1 expression affects human and mouse brain development

Weimin Bi, Tamar Sapir, Oleg A. Shchelochkov, Feng Zhang, Marjorie A. Withers, Jill V. Hunter, Talia Levy, Vera Shinder, Daniel A. Peiffer, Kevin L. Gunderson, Marjan M. Nezarati, Vern Ann Shotts, Stephen S. Amato, Sarah K. Savage, David J. Harris, Debra Lynn Day-Salvatore, Michele Horner, Xin Yan Lu, Trilochan Sahoo, Yuchio YanagawaArthur L. Beaudet, Sau Wai Cheung, Salvador Martinez, James R. Lupski, Orly Reiner

Research output: Contribution to journalArticle

165 Scopus citations

Abstract

Deletions of the PAFAH1B1 gene (encoding LIS1) in 17p13.3 result in isolated lissencephaly sequence, and extended deletions including the YWHAE gene (encoding 14-3-3ε) cause Miller-Dieker syndrome. We identified seven unrelated individuals with submicroscopic duplication in 17p13.3 involving the PAFAH1B1 and/or YWHAE genes, and using a 'reverse genomics' approach, characterized the clinical consequences of these duplications. Increased PAFAH1B1 dosage causes mild brain structural abnormalities, moderate to severe developmental delay and failure to thrive. Duplication of YWHAE and surrounding genes increases the risk for macrosomia, mild developmental delay and pervasive developmental disorder, and results in shared facial dysmorphologies. Transgenic mice conditionally overexpressing LIS1 in the developing brain showed a decrease in brain size, an increase in apoptotic cells and a distorted cellular organization in the ventricular zone, including reduced cellular polarity but preserved cortical cell layer identity. Collectively, our results show that an increase in LIS1 expression in the developing brain results in brain abnormalities in mice and humans.

Original languageEnglish (US)
Pages (from-to)168-177
Number of pages10
JournalNature Genetics
Volume41
Issue number2
DOIs
StatePublished - Feb 1 2009

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Increased LIS1 expression affects human and mouse brain development'. Together they form a unique fingerprint.

  • Cite this

    Bi, W., Sapir, T., Shchelochkov, O. A., Zhang, F., Withers, M. A., Hunter, J. V., Levy, T., Shinder, V., Peiffer, D. A., Gunderson, K. L., Nezarati, M. M., Ann Shotts, V., Amato, S. S., Savage, S. K., Harris, D. J., Day-Salvatore, D. L., Horner, M., Lu, X. Y., Sahoo, T., ... Reiner, O. (2009). Increased LIS1 expression affects human and mouse brain development. Nature Genetics, 41(2), 168-177. https://doi.org/10.1038/ng.302