Abstract
Vascular occlusion is more frequent in children with juvenile dermatomyositis (JDM) who have the TNFα-308A allele. One of the potent anti-angiogenic factors is thrombospondin-1 (TSP-1). This study investigated the association of the TNFα-308A allele with circulating levels of angiogenic mediators, TSP-1, and platelet factor 4 (PF4) using fresh, platelet-poor plasma (PPP). The TNFα-308A allele was characterized by PCR amplification and NcoI digestion. Concentrations of TSP-1 and PF4 in PPP from 31 JDM patients and 25 matched pediatric controls were determined by ELISA. The majority of the JDM children with the TNFα-308A allele (7/12) produced more TSP-1 than their TNFα-308G counterparts (P < 0.05), and their TSP-1 values were inversely related to those for PF4 (P < 0.0006). We conclude that the increased circulating concentrations of TSP-1 associated with the TNFα-308A allele suggest that this anti-angiogenic regulator may play a significant role in the augmented vascular occlusion observed in JDM children with this genetic marker.
Original language | English (US) |
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Pages (from-to) | 260-263 |
Number of pages | 4 |
Journal | Clinical Immunology |
Volume | 103 |
Issue number | 3 I |
DOIs | |
State | Published - 2002 |
Funding
Supported by grants from the National Institutes of Health RO1 AR43978 and P60 AR30692, The Marlene Apfelbaum Foundation and the Pappas Foundation.
Keywords
- And juvenile dermatomyositis
- Platelet factor 4
- TNFα-308A
- Thrombospondin-1
- Vascular occlusion
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology