Increased plasma thrombospondin-1 (TSP-1) levels are associated with the TNFα-308A allele in children with juvenile dermatomyositis

Jennica Lutz, Kristin G. Huwiler, Tamara Fedczyna, Toula S. Lechman, Susan Crawford, T. Randell Kinsella, Lauren M. Pachman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

33 Scopus citations

Abstract

Vascular occlusion is more frequent in children with juvenile dermatomyositis (JDM) who have the TNFα-308A allele. One of the potent anti-angiogenic factors is thrombospondin-1 (TSP-1). This study investigated the association of the TNFα-308A allele with circulating levels of angiogenic mediators, TSP-1, and platelet factor 4 (PF4) using fresh, platelet-poor plasma (PPP). The TNFα-308A allele was characterized by PCR amplification and NcoI digestion. Concentrations of TSP-1 and PF4 in PPP from 31 JDM patients and 25 matched pediatric controls were determined by ELISA. The majority of the JDM children with the TNFα-308A allele (7/12) produced more TSP-1 than their TNFα-308G counterparts (P < 0.05), and their TSP-1 values were inversely related to those for PF4 (P < 0.0006). We conclude that the increased circulating concentrations of TSP-1 associated with the TNFα-308A allele suggest that this anti-angiogenic regulator may play a significant role in the augmented vascular occlusion observed in JDM children with this genetic marker.

Original languageEnglish (US)
Pages (from-to)260-263
Number of pages4
JournalClinical Immunology
Volume103
Issue number3 I
DOIs
StatePublished - Jan 1 2002

Keywords

  • And juvenile dermatomyositis
  • Platelet factor 4
  • TNFα-308A
  • Thrombospondin-1
  • Vascular occlusion

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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