TY - JOUR
T1 - Increased survival of muscle stem cells lacking the MyoD gene after transplantation into regenerating skeletal muscle
AU - Asakura, Atsushi
AU - Hirai, Hiroyuki
AU - Kablar, Boris
AU - Morita, Shigeru
AU - Ishibashi, Jeff
AU - Piras, Bryan A.
AU - Christ, Amanda J.
AU - Verma, Mayank
AU - Vineretsky, Karin A.
AU - Rudnicki, Michael A.
PY - 2007/10/16
Y1 - 2007/10/16
N2 - MyoD is a myogenic master transcription factor that plays an essential role in muscle satellite cell (muscle stem cell) differentiation. To further investigate the function of MyoD in satellite cells, we examined the transplantation of satellite cell-derived myoblasts lacking the MyoD gene into regenerating skeletal muscle. After injection into injured muscle, MyoD -/- myoblasts engrafted with significantly higher efficiency compared with wild-type myoblasts. In addition, MyoD-/- myoblast-derived satellite cells were detected underneath the basal lamina of muscle fibers, indicating the self-renewal property of MyoD-/- myoblasts. To gain insights into MyoD gene deficiency in muscle stem cells, we investigated the pathways regulated by MyoD by GeneChip microarray analysis of gene expression in wild-type and MyoD-/- myoblasts. MyoD deficiency led to down-regulation of many muscle-specific genes and up-regulation of some stem cell markers. Importantly, in MyoD-/- myoblasts, many antiapoptotic genes were up-regulated, whereas genes known to execute apoptosis were down-regulated. Consistent with these gene expression profiles, MyoD -/- myoblasts were revealed to possess remarkable resistance to apoptosis and increased survival compared with wild-type myoblasts. Forced expression of MyoD or the proapoptotic protein Puma increased cell death in MyoD-/- myoblasts. Therefore, MyoD-/- myoblasts may preserve stem cell characteristics, including their resistance to apoptosis, expression of stem cell markers, and efficient engraftment and contribution to satellite cells after transplantation. Furthermore, our data offer evidence for improved therapeutic stem cell transplantation for muscular dystrophy, in which suppression of MyoD in myogenic progenitors would be beneficial to therapy by providing a selective advantage for the expansion of stem cells.
AB - MyoD is a myogenic master transcription factor that plays an essential role in muscle satellite cell (muscle stem cell) differentiation. To further investigate the function of MyoD in satellite cells, we examined the transplantation of satellite cell-derived myoblasts lacking the MyoD gene into regenerating skeletal muscle. After injection into injured muscle, MyoD -/- myoblasts engrafted with significantly higher efficiency compared with wild-type myoblasts. In addition, MyoD-/- myoblast-derived satellite cells were detected underneath the basal lamina of muscle fibers, indicating the self-renewal property of MyoD-/- myoblasts. To gain insights into MyoD gene deficiency in muscle stem cells, we investigated the pathways regulated by MyoD by GeneChip microarray analysis of gene expression in wild-type and MyoD-/- myoblasts. MyoD deficiency led to down-regulation of many muscle-specific genes and up-regulation of some stem cell markers. Importantly, in MyoD-/- myoblasts, many antiapoptotic genes were up-regulated, whereas genes known to execute apoptosis were down-regulated. Consistent with these gene expression profiles, MyoD -/- myoblasts were revealed to possess remarkable resistance to apoptosis and increased survival compared with wild-type myoblasts. Forced expression of MyoD or the proapoptotic protein Puma increased cell death in MyoD-/- myoblasts. Therefore, MyoD-/- myoblasts may preserve stem cell characteristics, including their resistance to apoptosis, expression of stem cell markers, and efficient engraftment and contribution to satellite cells after transplantation. Furthermore, our data offer evidence for improved therapeutic stem cell transplantation for muscular dystrophy, in which suppression of MyoD in myogenic progenitors would be beneficial to therapy by providing a selective advantage for the expansion of stem cells.
KW - Apoptosis
KW - Cell therapy
KW - Microarrays
KW - Muscular dystrophy
KW - Satellite cell
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U2 - 10.1073/pnas.0708145104
DO - 10.1073/pnas.0708145104
M3 - Article
C2 - 17940048
AN - SCOPUS:36749020558
SN - 0027-8424
VL - 104
SP - 16552
EP - 16557
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 42
ER -