Increased type I collagen gene expression in L-tryptophan associated eosinophilia-myalgia syndrome skin fibroblasts

J. Varga*, L. Li, S. A. Jimenez

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Objective. To investigate the pathogenetic mechanisms responsible for the cutaneous fibrosis in eosinophilia-myalgia syndrome (EMS) associated with L- tryptophan ingestion. Methods. Compare in vitro type I collagen production and steady state procollagen mRNA levels in cultured skin fibroblasts derived from healthy individuals and from 5 patients with EMS and diffuse cutaneous induration. Results. Cell lines derived from the affected skin from patients with EMS exhibited greater collagen production and higher steady state levels of α1(I) procollagen mRNA compared with fibroblasts from age and sex matched healthy individuals. Exposure to interferon gamma reduced collagen synthesis in the EMS fibroblast lines. The rate of in vitro transcription of the COL1A1 gene was 30% higher in nuclei isolated from collagen overproducer EMS fibroblasts than in nuclei from normal fibroblasts. Conclusion. Fibroblasts derived from the involved skin of patients with EMS show increased expression of the α1(I) procollagen gene in vitro compared to normal skin fibroblasts. The biosynthetically activated phenotype exhibited by EMS fibroblasts appears to be due, at least in part, to transcriptional activation of type I collagen gene expression. These biochemical and molecular alterations may result in accumulation of collagen and lead to the cutaneous fibrosis in EMS.

Original languageEnglish (US)
Pages (from-to)1303-1308
Number of pages6
JournalJournal of Rheumatology
Volume20
Issue number8
StatePublished - 1993

Keywords

  • COLLAGEN REGULATION
  • EOSINOPHILIA-MYALGIA SYNDROME
  • FIBROSIS

ASJC Scopus subject areas

  • Rheumatology
  • Immunology and Allergy
  • Immunology

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