Independent movement, dimerization and stability of tandem repeats of chicken brain α-spectrin

Hideki Kusunoki, George Minasov, Ruby I. MacDonald, Alfonso Mondragón

Research output: Contribution to journalArticle

86 Scopus citations

Abstract

Previous X-ray crystal structures have shown that linkers of five amino acid residues connecting pairs of chicken brain α-spectrin and human erythroid β-spectrin repeats can undergo bending without losing their α-helical structure. To test whether bending at one linker can influence bending at an adjacent linker, the structures of two and three repeat fragments of chicken brain α-spectrin have been determined by X-ray crystallography. The structure of the three-repeat fragment clearly shows that bending at one linker can occur independently of bending at an adjacent linker. This observation increases the possible trajectories of modeled chains of spectrin repeats. Furthermore, the three-repeat molecule crystallized as an antiparallel dimer with a significantly smaller buried interfacial area than that of α-actinin, a spectrin-related molecule, but large enough and of a type indicating biological specificity. Comparison of the structures of the spectrin and α-actinin dimers supports weak association of the former, which could not be detected by analytical ultracentrifugation, versus strong association of the latter, which has been observed by others. To correlate features of the structure with solution properties and to test a previous model of stable spectrin and dystrophin repeats, the number of inter-helical interactions in each repeat of several spectrin structures were counted and compared to their thermal stabilities. Inter-helical interactions, but not all interactions, increased in parallel with measured thermal stabilities of each repeat and in agreement with the thermal stabilities of two and three repeats and also partial repeats of spectrin.

Original languageEnglish (US)
Pages (from-to)495-511
Number of pages17
JournalJournal of Molecular Biology
Volume344
Issue number2
DOIs
StatePublished - Nov 19 2004

Keywords

  • crystal structure
  • dimer conformation
  • flexibility
  • spectrin
  • thermodynamic folding stability

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Fingerprint Dive into the research topics of 'Independent movement, dimerization and stability of tandem repeats of chicken brain α-spectrin'. Together they form a unique fingerprint.

  • Cite this