Indirect immunofluorescent assays for acute lymphoblastic leukemia (ALL) cell-associated antigen. Elimination of nonspecific fluorescent stain on lymphoid cells

C. C S Hsu, E. R. Morgan

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Abstract

Seventy-six children with acute lymphoblastic leukemia (ALL) were examined for T- and B-cell markers (sheep erythrocyte receptors and surface immunoglobulins, respectively) on leukemic cells. B-cell-associated or Ia-like antigen (Ia) and ALL-associated antigen (ALLA) were also assayed with indirect immunofluorescent technic using anti-EP and anti-ALL respectively. Four patients were found to have T-cell ALL; one patient was not classifiable, owing to changing expression of markers. Seventy-one patients had null-cell ALL without the T- or B-cell marker; 58 of these patients were ALLA positive (+). ALLA(+) patients were invariably Ia(+). The percentage of ALLA(+) cells closely correlated with the percentage of blast cells in the blood. Thirty-nine healthy subjects, 38 patients without leukemia and 54 patients with other leukemias were studied as negative controls for anti-ALL. Cells from some control subjects showed anti-ALL reactivity. Their cells also demonstrated nonspecific stains in the indirect immunofluorescent technic when normal rabbit serum was used instead of anti-ALL. Preincubating cells with human IgG and goat antihuman IgG eliminated the nonspecific stain on lymphoid cells, presumably by preempting Fc-receptors on the cell surface. With incorporation of this step to the immunofluorescent technic, mononuclear cells from the control subjects and marrow cells from patients with ALL in remission did not react with the anti-ALL. Enumeration of anti-ALL cells may be used to monitor disease activity in patients with ALL.

Original languageEnglish (US)
Pages (from-to)633-638
Number of pages6
JournalAmerican Journal of Clinical Pathology
Volume73
Issue number5
DOIs
StatePublished - Jan 1 1980

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Precursor Cell Lymphoblastic Leukemia-Lymphoma
Coloring Agents
Lymphocytes
Antigens
B-Lymphocytes
Leukemia
Immunoglobulin G
Precursor T-Cell Lymphoblastic Leukemia-Lymphoma
Null Lymphocytes
B-Cell Antigen Receptors
Fc Receptors
Histocompatibility Antigens Class II
Goats
Blood Cells
Sheep
Healthy Volunteers
Erythrocytes
Bone Marrow
Rabbits

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

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abstract = "Seventy-six children with acute lymphoblastic leukemia (ALL) were examined for T- and B-cell markers (sheep erythrocyte receptors and surface immunoglobulins, respectively) on leukemic cells. B-cell-associated or Ia-like antigen (Ia) and ALL-associated antigen (ALLA) were also assayed with indirect immunofluorescent technic using anti-EP and anti-ALL respectively. Four patients were found to have T-cell ALL; one patient was not classifiable, owing to changing expression of markers. Seventy-one patients had null-cell ALL without the T- or B-cell marker; 58 of these patients were ALLA positive (+). ALLA(+) patients were invariably Ia(+). The percentage of ALLA(+) cells closely correlated with the percentage of blast cells in the blood. Thirty-nine healthy subjects, 38 patients without leukemia and 54 patients with other leukemias were studied as negative controls for anti-ALL. Cells from some control subjects showed anti-ALL reactivity. Their cells also demonstrated nonspecific stains in the indirect immunofluorescent technic when normal rabbit serum was used instead of anti-ALL. Preincubating cells with human IgG and goat antihuman IgG eliminated the nonspecific stain on lymphoid cells, presumably by preempting Fc-receptors on the cell surface. With incorporation of this step to the immunofluorescent technic, mononuclear cells from the control subjects and marrow cells from patients with ALL in remission did not react with the anti-ALL. Enumeration of anti-ALL cells may be used to monitor disease activity in patients with ALL.",
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