Induction of allergic rhinitis in mice

Virginia Mc Millan Carr, Alan Robinson

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

We describe a method for allergic rhinitis (AR) induction in mice. Methodology involves nasal infusions of small volumes of ovalbumin for both initial sensitization and challenges. The latter are frequent and carried out over several weeks. This methodology more closely resembles natural AR induction than does the common use of systemic sensitization, often with adjuvants, followed by nasal challenges with relatively large allergen volumes. Also described are methodologies for collection of cardiac blood and perfusion for preparation of histological samples, both essential in verifying AR induction in individual animals.

Original languageEnglish (US)
Title of host publicationMouse Models of Allergic Disease
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc
Pages145-158
Number of pages14
ISBN (Print)9781627034951
DOIs
StatePublished - Jan 1 2013

Publication series

NameMethods in Molecular Biology
Volume1032
ISSN (Print)1064-3745

Keywords

  • Allergic rhinitis induction
  • Cardiac blood collection
  • Eosinophils
  • Mouse perfusion
  • Murine allergic rhinitis model
  • Nasal sinuses
  • Nasal tissues
  • Olfactory epithelium
  • Ovalbumin
  • Respiratory epithelium

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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