Induction of demyelination by intraneural injection of antibodies against sulfoglucuronyl paragloboside

Yasuhiro Maeda; John W. Bigbee; Reiko Maeda; Nobuyuki Miyatani; Robert G. Kalb; Robert K. Yu

doi:10.1016/0014-4886(91)90178-F

Induction of demyelination by intraneural injection of antibodies against sulfoglucuronyl paragloboside

Yasuhiro Maeda^*, John W. Bigbee, Reiko Maeda, Nobuyuki Miyatani, Robert G. Kalb, Robert K. Yu

^*Corresponding author for this work

Neurology

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

Sulfoglucuronyl glycolipids (SGGLs) carry the glucuronyl 3-sulfate (HNK-1) epitope which is recognized by monoclonal IgM paraproteins from patients with demyelinating polyneuropathy. We report that intraneural injections of rat anti-SGGL antibodies induce demyelination in rat sciatic nerve, along with mild to moderate clinical symptoms. Morphologically, vesiculation and loosening of the myelin sheath were observed 3 h postinjection, followed by extensive demyelination and macrophage infiltration after 4 days. Since the antiSGGL antibodies showed no cross-reactivity with other components in rat sciatic nerve, these results indicate that SGGLs alone can serve as the target antigens in demyelinating neuropathy.

Original language	English (US)
Pages (from-to)	221-225
Number of pages	5
Journal	Experimental Neurology
Volume	113
Issue number	2
DOIs	https://doi.org/10.1016/0014-4886(91)90178-F
State	Published - Aug 1991

Funding

We thank Dr. T. Saida, Kyoto, Japan, for technical advice. This work was supported by USPHS Grant NS-26994 and in part by Fidia Research Laboratories.

ASJC Scopus subject areas

Neurology
Developmental Neuroscience

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10.1016/0014-4886(91)90178-F

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title = "Induction of demyelination by intraneural injection of antibodies against sulfoglucuronyl paragloboside",

abstract = "Sulfoglucuronyl glycolipids (SGGLs) carry the glucuronyl 3-sulfate (HNK-1) epitope which is recognized by monoclonal IgM paraproteins from patients with demyelinating polyneuropathy. We report that intraneural injections of rat anti-SGGL antibodies induce demyelination in rat sciatic nerve, along with mild to moderate clinical symptoms. Morphologically, vesiculation and loosening of the myelin sheath were observed 3 h postinjection, followed by extensive demyelination and macrophage infiltration after 4 days. Since the antiSGGL antibodies showed no cross-reactivity with other components in rat sciatic nerve, these results indicate that SGGLs alone can serve as the target antigens in demyelinating neuropathy.",

author = "Yasuhiro Maeda and Bigbee, {John W.} and Reiko Maeda and Nobuyuki Miyatani and Kalb, {Robert G.} and Yu, {Robert K.}",

note = "Funding Information: We thank Dr. T. Saida, Kyoto, Japan, for technical advice. This work was supported by USPHS Grant NS-26994 and in part by Fidia Research Laboratories.",

year = "1991",

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doi = "10.1016/0014-4886(91)90178-F",

language = "English (US)",

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T1 - Induction of demyelination by intraneural injection of antibodies against sulfoglucuronyl paragloboside

AU - Maeda, Yasuhiro

AU - Bigbee, John W.

AU - Maeda, Reiko

AU - Miyatani, Nobuyuki

AU - Kalb, Robert G.

AU - Yu, Robert K.

N1 - Funding Information: We thank Dr. T. Saida, Kyoto, Japan, for technical advice. This work was supported by USPHS Grant NS-26994 and in part by Fidia Research Laboratories.

PY - 1991/8

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N2 - Sulfoglucuronyl glycolipids (SGGLs) carry the glucuronyl 3-sulfate (HNK-1) epitope which is recognized by monoclonal IgM paraproteins from patients with demyelinating polyneuropathy. We report that intraneural injections of rat anti-SGGL antibodies induce demyelination in rat sciatic nerve, along with mild to moderate clinical symptoms. Morphologically, vesiculation and loosening of the myelin sheath were observed 3 h postinjection, followed by extensive demyelination and macrophage infiltration after 4 days. Since the antiSGGL antibodies showed no cross-reactivity with other components in rat sciatic nerve, these results indicate that SGGLs alone can serve as the target antigens in demyelinating neuropathy.

AB - Sulfoglucuronyl glycolipids (SGGLs) carry the glucuronyl 3-sulfate (HNK-1) epitope which is recognized by monoclonal IgM paraproteins from patients with demyelinating polyneuropathy. We report that intraneural injections of rat anti-SGGL antibodies induce demyelination in rat sciatic nerve, along with mild to moderate clinical symptoms. Morphologically, vesiculation and loosening of the myelin sheath were observed 3 h postinjection, followed by extensive demyelination and macrophage infiltration after 4 days. Since the antiSGGL antibodies showed no cross-reactivity with other components in rat sciatic nerve, these results indicate that SGGLs alone can serve as the target antigens in demyelinating neuropathy.

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Induction of demyelination by intraneural injection of antibodies against sulfoglucuronyl paragloboside

Abstract

Funding

ASJC Scopus subject areas

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