Induction of human cytotoxic T lymphocytes specific for prostate- specific antigen

Bao Hua Xue, Yong Zhang, Jeffrey A. Sosman, David J. Peace*

*Corresponding author for this work

Research output: Contribution to journalArticle

70 Scopus citations

Abstract

Prostate-specific antigen (PSA), a tissue-specific protein expressed by most adenocarcinomas of the prostate, might be a useful target for T-cell- mediated immunotherapy of prostate cancers. The current study examined whether it is possible to elicit human cytotoxic T lymphocytes (CTL) with specificity for PSA. A synthetic nonamer peptide, corresponding to residues 146-154 of PSA and containing a canonical HLA-A2-binding motif, was shown to stabilize the expression of HLA-A2 on the T2 antigen-processing mutant cell line. Repeated in vitro stimulation of peripheral blood lymphocytes from a normal HLA-A2+ donor induced CTL with specificity for the PSA 146-154 peptide. The peptide-induced CTL expressed the CD4- CD8+ cell surface phenotype and were restricted by HLA-A2. A large portion of patients with prostate cancer express the HLA-A2 phenotype, implying that many prostate cancers might be targeted by HLA-A2-restricted CTL with specificity for the PSA 146-154 epitope.

Original languageEnglish (US)
Pages (from-to)73-78
Number of pages6
JournalProstate
Volume30
Issue number2
DOIs
StatePublished - Feb 1 1997

Keywords

  • HLA-A2
  • class I major histocompatibility complex
  • immunotherapy
  • in vitro immunization
  • tumor antigen

ASJC Scopus subject areas

  • Oncology
  • Urology

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