In this chapter, we will describe the use of a hypoxia chamber with a two-gas (carbon dioxide and nitrogen) supply in order to investigate the effects of low oxygen tension on angiogenesis. For this assay we describe the effects using human umbilical vein endothelial cells but in practice the protocol can be modified and optimized for use with any source of endothelial cells.
- Human umbilical vein endothelial cells
- Hypoxia inducible factor-1-alpha
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)