Induction of hypoxia in vascular endothelial cell culture

Hyun Young Koo, Meredith Millay, Tsutomu Kume*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter


In this chapter, we will describe the use of a hypoxia chamber with a two-gas (carbon dioxide and nitrogen) supply in order to investigate the effects of low oxygen tension on angiogenesis. For this assay we describe the effects using human umbilical vein endothelial cells but in practice the protocol can be modified and optimized for use with any source of endothelial cells.

Original languageEnglish (US)
Title of host publicationHandbook of Vascular Biology Techniques
PublisherSpringer Netherlands
Number of pages5
ISBN (Electronic)9789401797160
ISBN (Print)9789401797153
StatePublished - Jan 1 2015


  • Human umbilical vein endothelial cells
  • Hypoxia
  • Hypoxia inducible factor-1-alpha

ASJC Scopus subject areas

  • Medicine(all)
  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)


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