Induction of hypoxia in vascular endothelial cell culture

Hyun Young Koo; Meredith Millay; Tsutomu Kume

doi:10.1007/978-94-017-9716-0_3

Induction of hypoxia in vascular endothelial cell culture

Hyun Young Koo, Meredith Millay, Tsutomu Kume^*

^*Corresponding author for this work

Medicine, Cardiology Division

Research output: Chapter in Book/Report/Conference proceeding › Chapter

Abstract

In this chapter, we will describe the use of a hypoxia chamber with a two-gas (carbon dioxide and nitrogen) supply in order to investigate the effects of low oxygen tension on angiogenesis. For this assay we describe the effects using human umbilical vein endothelial cells but in practice the protocol can be modified and optimized for use with any source of endothelial cells.

Original language	English (US)
Title of host publication	Handbook of Vascular Biology Techniques
Publisher	Springer Netherlands
Pages	23-27
Number of pages	5
ISBN (Electronic)	9789401797160
ISBN (Print)	9789401797153
DOIs	https://doi.org/10.1007/978-94-017-9716-0_3
State	Published - Jan 1 2015

Keywords

Human umbilical vein endothelial cells
Hypoxia
Hypoxia inducible factor-1-alpha

ASJC Scopus subject areas

General Medicine
General Agricultural and Biological Sciences
General Biochemistry, Genetics and Molecular Biology

Access to Document

10.1007/978-94-017-9716-0_3

Cite this

@inbook{425454a57ea344e3808a5689f5e808df,

title = "Induction of hypoxia in vascular endothelial cell culture",

abstract = "In this chapter, we will describe the use of a hypoxia chamber with a two-gas (carbon dioxide and nitrogen) supply in order to investigate the effects of low oxygen tension on angiogenesis. For this assay we describe the effects using human umbilical vein endothelial cells but in practice the protocol can be modified and optimized for use with any source of endothelial cells.",

keywords = "Human umbilical vein endothelial cells, Hypoxia, Hypoxia inducible factor-1-alpha",

author = "Koo, {Hyun Young} and Meredith Millay and Tsutomu Kume",

note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media Dordrecht 2015.",

year = "2015",

month = jan,

day = "1",

doi = "10.1007/978-94-017-9716-0_3",

language = "English (US)",

isbn = "9789401797153",

pages = "23--27",

booktitle = "Handbook of Vascular Biology Techniques",

publisher = "Springer Netherlands",

}

TY - CHAP

T1 - Induction of hypoxia in vascular endothelial cell culture

AU - Koo, Hyun Young

AU - Millay, Meredith

AU - Kume, Tsutomu

N1 - Publisher Copyright: © Springer Science+Business Media Dordrecht 2015.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - In this chapter, we will describe the use of a hypoxia chamber with a two-gas (carbon dioxide and nitrogen) supply in order to investigate the effects of low oxygen tension on angiogenesis. For this assay we describe the effects using human umbilical vein endothelial cells but in practice the protocol can be modified and optimized for use with any source of endothelial cells.

AB - In this chapter, we will describe the use of a hypoxia chamber with a two-gas (carbon dioxide and nitrogen) supply in order to investigate the effects of low oxygen tension on angiogenesis. For this assay we describe the effects using human umbilical vein endothelial cells but in practice the protocol can be modified and optimized for use with any source of endothelial cells.

KW - Human umbilical vein endothelial cells

KW - Hypoxia

KW - Hypoxia inducible factor-1-alpha

UR - http://www.scopus.com/inward/record.url?scp=84943163462&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84943163462&partnerID=8YFLogxK

U2 - 10.1007/978-94-017-9716-0_3

DO - 10.1007/978-94-017-9716-0_3

M3 - Chapter

AN - SCOPUS:84943163462

SN - 9789401797153

SP - 23

EP - 27

BT - Handbook of Vascular Biology Techniques

PB - Springer Netherlands

ER -

Induction of hypoxia in vascular endothelial cell culture

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this