Induction of spine growth and synapse formation by regulation of the spine actin cytoskeleton

Karen Zito; Graham Knott; Gordon M G Shepherd; Shirish Shenolikar; Karel Svoboda

doi:10.1016/j.neuron.2004.09.022

Induction of spine growth and synapse formation by regulation of the spine actin cytoskeleton

Karen Zito, Graham Knott, Gordon M G Shepherd, Shirish Shenolikar, Karel Svoboda^*

^*Corresponding author for this work

Physiology

Research output: Contribution to journal › Article

151 Scopus citations

Abstract

We explored the relationship between regulation of the spine actin cytoskeleton, spine morphogenesis, and synapse formation by manipulating expression of the actin binding protein NrbI and its deletion mutants. In pyramidal neurons of cultured rat hippocampal slices, NrbI is concentrated in dendritic spines by binding to the actin cytoskeleton. Expression of one NrbI deletion mutant, containing the actin binding domain, dramatically increased the density and length of dendritic spines with synapses. This hyperspinogenesis was accompanied by enhanced actin polymerization and spine motility. Synaptic strengths were reduced to compensate for extra synapses, keeping total synaptic input per neuron constant. Our data support a model in which synapse formation is promoted by actin-powered motility.

Original language	English (US)
Pages (from-to)	321-334
Number of pages	14
Journal	Neuron
Volume	44
Issue number	2
DOIs	https://doi.org/10.1016/j.neuron.2004.09.022
State	Published - Oct 14 2004

ASJC Scopus subject areas

Neuroscience(all)

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Zito, K., Knott, G., Shepherd, G. M. G., Shenolikar, S., & Svoboda, K. (2004). Induction of spine growth and synapse formation by regulation of the spine actin cytoskeleton. Neuron, 44(2), 321-334. https://doi.org/10.1016/j.neuron.2004.09.022

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