Induction of T cell serine proteinase 1 (TSP‐1)‐specific mRNA in mouse T lymphocytes

Hans‐Georg ‐G Simon, Uli Fruth, Christoph Eckerskorn, Friedrich Lottspeich, Michael D. Kramer, Gabi Nerz, Markus M. Simon*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

An oligonucleotide probe corresponding to nucleotides of a cDNA encoding the T cell‐associated proteinase 1 (TSP‐1) was chosen to study the induction and expression of TSP‐1‐specific transcripts in mouse T lymphocytes and tissues. We demonstrate that TSP‐1 mRNA is only expressed in activated T lymphocytes and is absent from all mouse tissues tested including those containing resting mature T lymphocytes. Expression of the TSP‐1 gene was observed in T lymphoctes in vitro in response to either phorbolester (phorbol 12‐myristate 13‐acetate), Ca2+ ionophore (A23187), lectin or alloantigen. In general, TSP‐1 mRNA appeared and peaked later compared to interleukin 2 transcripts. Furthermore, TSP‐1 mRNA was inducible in vitro in both Ly‐2+ and L3T4+ lymphocyte populations treated with alloantigen and/or lectin. The transcription of the TSP‐1 gene was always accompanied by the expression of proteinase activity. High expression of TSP‐1 transcripts was also observed in in vivo derived T effector cells specific for lymphocytic choriomeningitis virus. However, TSP‐1 mRNA was predominantly associated with virus‐specific Ly‐2+ T cells and correlated with their proteinase and cytolytic activities. The data suggest that TSP‐1 gene transcription is a useful marker to characterize T effector cells in vitro and in vivo.

Original languageEnglish (US)
Pages (from-to)855-861
Number of pages7
JournalEuropean Journal of Immunology
Volume18
Issue number6
DOIs
StatePublished - Jun 1988

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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